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Sample GSM846042 Query DataSets for GSM846042
Status Public on Feb 22, 2012
Title MLL1_mouse_ChIPSeq
Sample type SRA
Source name MLL-AF9 transfromed blasts
Organism Mus musculus
Characteristics strain: C57BL/6
cell origin: 5-FU treated BM cells transduced with Flag-tagged MLL-AF9
chip antibody: anti-MLL1
Treatment protocol Cells were subjected to ChIP-Seq using an antibody for MLL1
Growth protocol Murine bone marrow cells were transformed with Flag-tagged MLL-AF9 , harvested after two rounds of replating.
Extracted molecule genomic DNA
Extraction protocol ChIP DNA libraries were made following Illumina ChIP-seq library preparation kit
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
Description Chromatin IP using anti-MLL1 antibody
Data processing Sequence reads were aligned to mouse genome assembly mm8 using Bowtie. ChIP-Seq signal was quantified as total number of reads per million in the region of interest. An empirical background distribution model of reads was constructed to find the significance level of signal in a given region.
Submission date Dec 08, 2011
Last update date May 15, 2019
Contact name Amit Sinha
Phone 617-582-7579
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Armstrong Lab
Street address 44 Binney St
City Boston
State/province MA
ZIP/Postal code 02135
Country USA
Platform ID GPL11002
Series (1)
GSE34261 ChipSeq of MLL1 in mouse leukemic blast cells
SRA SRX110650
BioSample SAMN00764093

Supplementary file Size Download File type/resource
GSM846042_MLL_Nter.a_m_1_best_strata.mm8.wig.gz 13.5 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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