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Sample GSM84597 Query DataSets for GSM84597
Status Public on Feb 05, 2006
Title HeLa_TP082505-32_HG-U133A_2_IMFGN0023
Sample type RNA
 
Source name Sample Type: Cells, Cell Line: HeLa, ATCC Number: CCL-2, Number of Cells Seeded: 2.20E+05, Cell Type: Cervical Carcinoma, Separation Type: Trypsin, Harvest Time Point: 12, Units of Harvest Time Point: hr
Organism Homo sapiens
Characteristics Array Type: HG-U133A_2
Ratio: 2.02
RNA Integrity Number: 9.6
Bioanalyzer Results: Excellent
Sample Preservation: -70C
Biomaterial provider Todd Page, CIIT Centers for Health Research, 6 Davis Dr, Durham, NC, 27709, T. Page, tpage@ciit.org, 919 558 1200, 919 558 1300
Treatment protocol in vitro Treatment: siLuc (Sense 5'-rCrGUrArCrGrCrGrGrArAUrArCUUrCrGrCTT-3’ Antisense 5’-UrCrGrArArGUrAUUrCrCrGrCrGUrArCrGTT-3’), Treatment Repeat: 1, Units of Treatment Repeat: dose, Treatment Duration: 60, Units of Duration: hrs, Treatment Time: -48, Units of Treatment Time: hrs, Vehicle for Chemical: Lipofectamine`, Amount of Vehicle: 0.44, Route of Exposure: Transfection, Units of Amount of Vehicle: microliter, Dose or Concentration: 3.3, Units of Dose or Concentration: nM
Treatment_2: in vitro Treatment: Heat, 43ºC, Treatment Repeat: 1, Units of Treatment Repeat: exposure, Treatment Duration: 1.5, Units of Duration: hrs, Treatment Time: 0, Units of Treatment Time: hrs, Route of Exposure: Convection, Dose or Concentration: 43, Units of Dose or Concentration: degrees Celcius
Growth protocol Growth Media: DMEM_10%FBS_P/S
Amount of Media: 5
Units of Media: ml
Growth Conditions: 37 C_5% CO2
Extracted molecule total RNA
Extraction protocol Extraction Method: Qiagen_RNeasy_Kit
Label biotin
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description TP082505-32_HG-U133A_2_IMFGN0023: HeLa cells were transfected with siRNA 48 hrs prior to heat shock. At time=0 HeLa cells were placed in a 43C incubator for 1.5 hrs, at the indicated time points RNA was harvested and Affymetrix microarrays were run.
Data processing affylmGUI, The Walter and Eliza Hall Institute of Medical Research
 
Submission date Nov 22, 2005
Last update date Apr 18, 2006
Contact name Todd Page
E-mail(s) tpage@ciit.org
Phone 919-558-1372
Organization name CIIT
Street address 6 Davis Dr
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL571
Series (1)
GSE3697 Treatment of heat shocked HeLa cells with siRNA (siHSF1#1)

Data table header descriptions
ID_REF Affymetrix probe name
VALUE RMA Expressioni Value

Data table
ID_REF VALUE
1007_s_at 9.067713505
1053_at 9.804914998
117_at 6.065029377
121_at 7.432033865
1255_g_at 3.558541607
1294_at 5.417902867
1316_at 5.267472311
1320_at 5.479428787
1405_i_at 3.781994927
1431_at 3.925142617
1438_at 5.302852272
1487_at 8.649751816
1494_f_at 5.194268007
1598_g_at 8.287871472
160020_at 6.40528341
1729_at 7.460617575
177_at 5.704495529
1773_at 6.730710105
179_at 8.881236424
1861_at 7.752790928

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary data files not provided

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