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Sample GSM84575 Query DataSets for GSM84575
Status Public on Feb 05, 2006
Title HeLa_06_21_04 TP-7
Sample type RNA
 
Source name Sample Type: Cells, Cell Line: HeLa, ATCC Number: CCL-2, Number of Cells Seeded: 2.20E+05, Cell Type: cervical carcinoma, Separation Type: Trypsin, Harvest Time Point: 2, Units of Harvest Time Point: hr
Organism Homo sapiens
Characteristics Array Type: HG-U133A_2
Ratio: 2.02
Bioanalyzer Results: Excellent
Sample Preservation: -70C
Biomaterial provider Todd Page, CIIT Centers for Health Research, 6 Davis Dr, Durham, NC, 27709, T. Page, tpage@ciit.org, 919 558 1200, 919 558 1300
Treatment protocol in vitro Treatment: siHSF1_#1 (Sense 5’-rGUrCrGUrCrArArCrArArGrCUrCrAUUrATT-3' Antisense 5’rGrArAUrGrArGrCUUrGUUrGrArCrGrArCTT-3’), Treatment Repeat: 1, Units of Treatment Repeat: dose, Treatment Duration: 50, Units of Duration: hours, Treatment Time: -48, Units of Treatment Time: hrs, Vehicle for Chemical: Lipofectamine 2000, Amount of Vehicle: 0.44, Route of Exposure: transfection, Units of Amount of Vehicle: ul, Dose or Concentration: 3.3, Units of Dose or Concentration: nM
Treatment_2: in vitro Treatment: Heat, 43ºC, Treatment Repeat: 1, Units of Treatment Repeat: exposure, Treatment Duration: 1.5, Units of Duration: hours, Treatment Time: 0, Units of Treatment Time: hrs, Route of Exposure: convection, Dose or Concentration: 43, Units of Dose or Concentration: degrees Celcius
Growth protocol Growth Media: DMEM_10%FBS_P/S
Amount of Media: 5
Units of Media: ml
Growth Conditions: 37 C 5% CO2
Extracted molecule total RNA
Extraction protocol Extraction Method: Qiagen_Rneasy_Kit
Label biotin
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description 06_21_04 TP-7: HeLa cells were transfected with siRNA 48 hrs prior to heat shock. At time=0 HeLa cells were placed in a 43C incubator for 1.5 hrs, at the indicated time points RNA was harvested and Affymetrix microarrays were run.
Data processing affylmGUI, The Walter and Eliza Hall Institute of Medical Research
 
Submission date Nov 22, 2005
Last update date Nov 30, 2005
Contact name Todd Page
E-mail(s) tpage@ciit.org
Phone 919-558-1372
Organization name CIIT
Street address 6 Davis Dr
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL571
Series (1)
GSE3697 Treatment of heat shocked HeLa cells with siRNA (siHSF1#1)

Data table header descriptions
ID_REF Affymetrix probe name
VALUE RMA Expressioni Value

Data table
ID_REF VALUE
1007_s_at 9.496243712
1053_at 9.701961304
117_at 9.642301035
121_at 7.276135197
1255_g_at 3.685699977
1294_at 5.262979171
1316_at 5.152878131
1320_at 5.479259312
1405_i_at 3.511122119
1431_at 3.800540976
1438_at 5.456336863
1487_at 8.322143846
1494_f_at 5.061229016
1598_g_at 8.033896122
160020_at 6.23757105
1729_at 7.560474781
177_at 6.142383476
1773_at 6.708355909
179_at 8.742519933
1861_at 7.499463386

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary data files not provided

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