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| Status |
Public on Aug 09, 2024 |
| Title |
Sample2_Antibody_Capture |
| Sample type |
SRA |
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| Source name |
Spleen
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| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
cell line: Primary cell
cell type: Tfh (CD4+CD44+PD-1+CXCR5+)
library type: ADT
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| Extracted molecule |
total RNA |
| Extraction protocol |
Splenocytes were incubated with antibody cocktail (including Hashtag antibodies), and Tfh cells were FACS-purified. After counting, Tfh cells were pooled, resuspend in sorting buffer (2% FBS in DPBS), and loaded onto the 10X Chromium.
Library construction was performed according to manufacturer's instruction (10X Genomics)
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| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
10X Genomics
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| Data processing |
Cellranger software
Assembly: mm10
Supplementary files format and content: Default outputs from Cellranger
Library strategy: scADT-seq
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| Submission date |
Aug 08, 2024 |
| Last update date |
Aug 09, 2024 |
| Contact name |
Xin Gao |
| E-mail(s) |
xin.gao@anu.edu.au
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| Phone |
0450031169
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| Organization name |
The Australian National University
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| Department |
Immunology
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| Street address |
131 Garran Rd
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| City |
Canberra |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE274341 |
B cells targeting parasites capture spatially linked antigens to secure T cell help |
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| Relations |
| BioSample |
SAMN43082669 |
| SRA |
SRX25643340 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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