|
| Status |
Public on Aug 09, 2024 |
| Title |
Sample2_Transcriptomes |
| Sample type |
SRA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
cell line: Primary cell
cell type: Tfh (CD4+CD44+PD-1+CXCR5+)
library type: GEX
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Splenocytes were incubated with antibody cocktail (including Hashtag antibodies), and Tfh cells were FACS-purified. After counting, Tfh cells were pooled, resuspend in sorting buffer (2% FBS in DPBS), and loaded onto the 10X Chromium.
Library construction was performed according to manufacturer's instruction (10X Genomics)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10X Genomics
|
| Data processing |
Cellranger software
Assembly: mm10
Supplementary files format and content: Default outputs from Cellranger
|
| |
|
| Submission date |
Aug 08, 2024 |
| Last update date |
Aug 09, 2024 |
| Contact name |
Xin Gao |
| E-mail(s) |
xin.gao@anu.edu.au
|
| Phone |
0450031169
|
| Organization name |
The Australian National University
|
| Department |
Immunology
|
| Street address |
131 Garran Rd
|
| City |
Canberra |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE274341 |
B cells targeting parasites capture spatially linked antigens to secure T cell help |
|
| Relations |
| BioSample |
SAMN43082670 |
| SRA |
SRX25643341 |
