|
| Status |
Public on Mar 21, 2012 |
| Title |
DAC Treated Primary Leukemia 1307 - Day 3 |
| Sample type |
genomic |
| |
|
| Source name |
Primary Leukemia
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Primary Cancer
cell type: leukemia cells
treatement: DAC
time: day 3
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Phenol-chloroform extraction
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted (EZ DNA methylation kit) DNA was amplified, fragmented and hybridised to Illumina HumanMethylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
Bisulfite treated genomic DNA
DAC treated primary leukemia, 3 days post treatment
X1307_DAC_d3
|
| Data processing |
Raw methylated and unmethylated signals are used to recalculate beta values without background subtraction. Probes with a detection p-value equal to or greater than .05 are set to NA. Data handling and processing done in R.
|
| |
|
| Submission date |
Dec 06, 2011 |
| Last update date |
Jul 24, 2013 |
| Contact name |
Leander Van Neste |
| Organization name |
Ghent University
|
| Department |
Molecular Biotechnology
|
| Lab |
Bioinformatics and Computational Genomics
|
| Street address |
Coupure Links 653
|
| City |
Ghent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
|
