|
Status |
Public on Dec 06, 2011 |
Title |
lung_tumor_resection_1418T |
Sample type |
genomic |
|
|
Source name |
lung tumor
|
Organism |
Homo sapiens |
Characteristics |
smoking packyears: 30 Stage: 1 histology: 0
|
Treatment protocol |
None
|
Growth protocol |
None
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted from tumor tissue from non-small cell lung cancer patients at surgery using the Promega Qiagen DNA Extraction Kit. DNA quality and quantity was assessed using OD measure and agarose gel electrophoresis.
|
Label |
biotin
|
Label protocol |
As per manufacturer (Affymetrix)
|
|
|
Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
Description |
lung_tumor_resection_rep1
|
Data processing |
CEL files and TXT files (generated by Affymetrix GTYPE using the DM algorithm) were processed by dChip and model-based expression performed using the PM/MM model. The data table contains the normalized expression values produced by that algorithm.
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|
|
Submission date |
Dec 05, 2011 |
Last update date |
Dec 06, 2011 |
Contact name |
Yen-Tsung Huang |
Organization name |
Harvard School of Public Health
|
Street address |
665 Huntington Avenue
|
City |
Boston |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL3718 |
Series (1) |
GSE34140 |
Impact on Disease Development, Genomic Location and Biological Function of Copy Number Alterations in Non-Small Cell Lung Cancer |
|