|
| Status |
Public on Dec 06, 2011 |
| Title |
lung_tumor_resection_1384T |
| Sample type |
genomic |
| |
|
| Source name |
lung tumor
|
| Organism |
Homo sapiens |
| Characteristics |
smoking packyears: 59
Stage: 1
histology: 0
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from tumor tissue from non-small cell lung cancer patients at surgery using the Promega Qiagen DNA Extraction Kit. DNA quality and quantity was assessed using OD measure and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
lung_tumor_resection_rep1
|
| Data processing |
CEL files and TXT files (generated by Affymetrix GTYPE using the DM algorithm) were processed by dChip and model-based expression performed using the PM/MM model. The data table contains the normalized expression values produced by that algorithm.
|
| |
|
| Submission date |
Dec 05, 2011 |
| Last update date |
Dec 06, 2011 |
| Contact name |
Yen-Tsung Huang |
| Organization name |
Harvard School of Public Health
|
| Street address |
665 Huntington Avenue
|
| City |
Boston |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL3718 |
| Series (1) |
| GSE34140 |
Impact on Disease Development, Genomic Location and Biological Function of Copy Number Alterations in Non-Small Cell Lung Cancer |
|
