|
| Status |
Public on Jul 22, 2024 |
| Title |
Control mouse 2 |
| Sample type |
SRA |
| |
|
| Source name |
brain
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain
genotype: wild type
treatment: treated with an empty Adeno-associated-virus vector with no insert
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
The reads were aligned to the UCSC Mus musculis reference genome mm9 with TopHat version 2.0.4, and multi-aligned reads were removed with SAMtools.
We then used HTSeq to generate mapping counts for the known annotated genes
We used Cuffdiff to convert the counts into total FPKM values, which represented the gene expression levels.
DEGs between AC109347.1 overexpressed and wild type were identified with the edgeR ver. 2.6.12 statistical package.
Assembly: mm9
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample ...
|
| |
|
| Submission date |
Jul 22, 2024 |
| Last update date |
Jul 22, 2024 |
| Contact name |
Cunyou Zhao |
| E-mail(s) |
cyzhao@smu.edu.cn
|
| Organization name |
Southern Medical University
|
| Street address |
No. 1023-1063, South Sha-tai Road, Baiyun District
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510515 |
| Country |
China |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE272753 |
Gain of bipolar disorder-related lncRNA AP1AR-DT in mice induces depressive and anxiety-like behaviors by reducing Negr1-mediated excitatory synaptic transmission [Mouse RNA-seq] |
|
| Relations |
| BioSample |
SAMN42691655 |
| SRA |
SRX25401776 |
