|
Status |
Public on Dec 01, 2011 |
Title |
B6C3Fe a/a-bpck mutant |
Sample type |
RNA |
|
|
Source name |
B6C3Fe a/a-bpck mutant
|
Organism |
Mus musculus |
Characteristics |
tissue: kidney genotype/variation: B6C3Fe a/a-bpck mutant age: postnatal 3 days
|
Treatment protocol |
Animal care and experimental procedures conformed to National Institutes of Health guidelines and were approved by the Institutional Animal Care and Use Committee at the University of Louisville (protocol # 09014), Louisville, KY.
|
Growth protocol |
mice were maintained at the Research and Resource Center at University of Louisville.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA
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Hybridization protocol |
Following fragmentation,30μl of cRNA were hybridized for 16 hr at 45℃ on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the GeneArray Scanner 3000 7G 4C.
|
Description |
Mouse in fast phase of renal corpuscle proliferation
|
Data processing |
The data were analyzed with dChip (Dec.2010 version)
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Submission date |
Nov 30, 2011 |
Last update date |
Dec 07, 2011 |
Contact name |
E Du |
E-mail(s) |
duedoc@126.com
|
Organization name |
Tianjin institude of Urology
|
Street address |
ping jiang road 23#, Hexi district
|
City |
Tianjin |
ZIP/Postal code |
300211 |
Country |
China |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE34051 |
Expression profiling of the early postnatal stage of Polycystic Kidney Disease in the B6C3Fe a/a-bpck mice |
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