|
| Status |
Public on Dec 01, 2011 |
| Title |
B6C3Fe a/a-bpck mutant |
| Sample type |
RNA |
| |
|
| Source name |
B6C3Fe a/a-bpck mutant
|
| Organism |
Mus musculus |
| Characteristics |
tissue: kidney
genotype/variation: B6C3Fe a/a-bpck mutant
age: postnatal 3 days
|
| Treatment protocol |
Animal care and experimental procedures conformed to National Institutes of Health guidelines and were approved by the Institutional Animal Care and Use Committee at the University of Louisville (protocol # 09014), Louisville, KY.
|
| Growth protocol |
mice were maintained at the Research and Resource Center at University of Louisville.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation,30μl of cRNA were hybridized for 16 hr at 45℃ on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner 3000 7G 4C.
|
| Description |
Mouse in fast phase of renal corpuscle proliferation
|
| Data processing |
The data were analyzed with dChip (Dec.2010 version)
|
| |
|
| Submission date |
Nov 30, 2011 |
| Last update date |
Dec 07, 2011 |
| Contact name |
E Du |
| E-mail(s) |
duedoc@126.com
|
| Organization name |
Tianjin institude of Urology
|
| Street address |
ping jiang road 23#, Hexi district
|
| City |
Tianjin |
| ZIP/Postal code |
300211 |
| Country |
China |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE34051 |
Expression profiling of the early postnatal stage of Polycystic Kidney Disease in the B6C3Fe a/a-bpck mice |
|
