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Sample GSM840946 Query DataSets for GSM840946
Status Public on Nov 30, 2011
Title HepG2_Elaidic acid_rep3
Sample type RNA
Source name HepG2, Elaidic acid, 7 days
Organism Homo sapiens
Characteristics cell line: HepG2
incubated fatty acid: Elaidic acid
Treatment protocol Incubation for seven days with 100 µM fatty acid (oleic, elaidic or stearic acid)
Extracted molecule total RNA
Extraction protocol Total RNA Purification Kit (NORGEN BIOTEK CORPORATION) Distribubed by Phalanx Biotech
Label Cy5
Label protocol RNA was converted to double-stranded cDNA and amplified using an in vitro transcription reaction that included aminoallyl-UTP, and the aRNA product was conjugated with Cy5T NHS ester
Hybridization protocol Fragmented aRNA was hybridized at 42° C overnight using the HybBag mixing system with 1× OneArray Hybridization Buffer (Phalanx Biotech), 0.01 mg/mL sheared salmon sperm DNA (Promega) and 0.025 mg/mL labeled target. After hybridization, the arrays were washed according to the OneArray protocol
Scan protocol Raw intensity signals from each microarray were captured using a Molecular Dynamics™ Axon 4100A scanner, measured using GenePixPro™ Software, and stored in GPR format
Description EC
raw data file technical rep1: H021-0607002936.gpr
raw data file technical rep2: H022-0607002937.gpr
Data processing The data from all microarrays in each experimental set were passed to Rosetta Resolver (Rosetta Biosoftware) for analysis. Testing was performed by combining the technical replicates and performing statistical analyses using Rosetta Resolver's proprietary modeling techniques
Submission date Nov 30, 2011
Last update date Nov 30, 2011
Contact name Toke Krogager Hansen
Organization name Aarhus University
Department Molecular Biology
Lab Proteome analysis
Street address gustav wieds vej 10c
City Aarhus
ZIP/Postal code 8000
Country Denmark
Platform ID GPL6254
Series (1)
GSE34045 Identification of a biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Data table header descriptions
VALUE Normalized signal Intensity calculated by Rosetta Resolver

Data table
PH_hs_0000002 161.367523
PH_hs_0000003 12.6935625
PH_hs_0000004 133.508957
PH_hs_0000005 9.6420269
PH_hs_0000006 673.098633
PH_hs_0000007 564.564941
PH_hs_0000008 43.4285965
PH_hs_0000009 85.2507629
PH_hs_0000010 1566.57031
PH_hs_0000011 147.805511
PH_hs_0000012 239.567825
PH_hs_0000013 446.244995
PH_hs_0000014 753.718872
PH_hs_0000015 3578.53076
PH_hs_0000016 2850.97485
PH_hs_0000017 106.814026
PH_hs_0000018 33.8929787
PH_hs_0000019 13.7919779
PH_hs_0000020 6066.35938
PH_hs_0000021 147.197968

Total number of rows: 30968

Table truncated, full table size 754 Kbytes.

Supplementary file Size Download File type/resource
GSM840946_H021-0607002936.gpr.gz 2.6 Mb (ftp)(http) GPR
GSM840946_H022-0607002937.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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