GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM840944 Query DataSets for GSM840944
Status Public on Nov 30, 2011
Title HepG2_Elaidic acid_rep1
Sample type RNA
Source name HepG2, Elaidic acid, 7 days
Organism Homo sapiens
Characteristics cell line: HepG2
incubated fatty acid: Elaidic acid
Treatment protocol Incubation for seven days with 100 µM fatty acid (oleic, elaidic or stearic acid)
Extracted molecule total RNA
Extraction protocol Total RNA Purification Kit (NORGEN BIOTEK CORPORATION) Distribubed by Phalanx Biotech
Label Cy5
Label protocol RNA was converted to double-stranded cDNA and amplified using an in vitro transcription reaction that included aminoallyl-UTP, and the aRNA product was conjugated with Cy5T NHS ester
Hybridization protocol Fragmented aRNA was hybridized at 42° C overnight using the HybBag mixing system with 1× OneArray Hybridization Buffer (Phalanx Biotech), 0.01 mg/mL sheared salmon sperm DNA (Promega) and 0.025 mg/mL labeled target. After hybridization, the arrays were washed according to the OneArray protocol
Scan protocol Raw intensity signals from each microarray were captured using a Molecular Dynamics™ Axon 4100A scanner, measured using GenePixPro™ Software, and stored in GPR format
Description EA
raw data file technical rep1: H017-0607002932.gpr
raw data file technical rep2: H018-0607002933.gpr
Data processing The data from all microarrays in each experimental set were passed to Rosetta Resolver (Rosetta Biosoftware) for analysis. Testing was performed by combining the technical replicates and performing statistical analyses using Rosetta Resolver's proprietary modeling techniques
Submission date Nov 30, 2011
Last update date Nov 30, 2011
Contact name Toke Krogager Hansen
Organization name Aarhus University
Department Molecular Biology
Lab Proteome analysis
Street address gustav wieds vej 10c
City Aarhus
ZIP/Postal code 8000
Country Denmark
Platform ID GPL6254
Series (1)
GSE34045 Identification of a biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Data table header descriptions
VALUE Normalized signal Intensity calculated by Rosetta Resolver

Data table
PH_hs_0000002 154.480713
PH_hs_0000003 18.6692486
PH_hs_0000004 143.201141
PH_hs_0000005 -5.36082506
PH_hs_0000006 468.385498
PH_hs_0000007 700.680115
PH_hs_0000008 18.3805275
PH_hs_0000009 93.3364868
PH_hs_0000010 1575.8269
PH_hs_0000011 131.465057
PH_hs_0000012 215.437653
PH_hs_0000013 404.566711
PH_hs_0000014 751.959106
PH_hs_0000015 3007.3916
PH_hs_0000016 2418.04224
PH_hs_0000017 76.0074463
PH_hs_0000018 62.1781578
PH_hs_0000019 7.91053295
PH_hs_0000020 7254.91846
PH_hs_0000021 92.1445465

Total number of rows: 30968

Table truncated, full table size 754 Kbytes.

Supplementary file Size Download File type/resource
GSM840944_H017-0607002932.gpr.gz 2.6 Mb (ftp)(http) GPR
GSM840944_H018-0607002933.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap