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Sample GSM840943 Query DataSets for GSM840943
Status Public on Nov 30, 2011
Title HepG2_Oleic acid_rep4
Sample type RNA
Source name HepG2, Oleic acid, 7 days
Organism Homo sapiens
Characteristics cell line: HepG2
incubated fatty acid: Oleic acid
Treatment protocol Incubation for seven days with 100 µM fatty acid (oleic, elaidic or stearic acid)
Extracted molecule total RNA
Extraction protocol Total RNA Purification Kit (NORGEN BIOTEK CORPORATION) Distribubed by Phalanx Biotech
Label Cy5
Label protocol RNA was converted to double-stranded cDNA and amplified using an in vitro transcription reaction that included aminoallyl-UTP, and the aRNA product was conjugated with Cy5T NHS ester
Hybridization protocol Fragmented aRNA was hybridized at 42° C overnight using the HybBag mixing system with 1× OneArray Hybridization Buffer (Phalanx Biotech), 0.01 mg/mL sheared salmon sperm DNA (Promega) and 0.025 mg/mL labeled target. After hybridization, the arrays were washed according to the OneArray protocol
Scan protocol Raw intensity signals from each microarray were captured using a Molecular Dynamics™ Axon 4100A scanner, measured using GenePixPro™ Software, and stored in GPR format
Description OD
raw data file technical rep1: H015-0607008712.gpr
raw data file technical rep2: H016-0607008713.gpr
Data processing The data from all microarrays in each experimental set were passed to Rosetta Resolver (Rosetta Biosoftware) for analysis. Testing was performed by combining the technical replicates and performing statistical analyses using Rosetta Resolver's proprietary modeling techniques
Submission date Nov 30, 2011
Last update date Nov 30, 2011
Contact name Toke Krogager Hansen
Organization name Aarhus University
Department Molecular Biology
Lab Proteome analysis
Street address gustav wieds vej 10c
City Aarhus
ZIP/Postal code 8000
Country Denmark
Platform ID GPL6254
Series (1)
GSE34045 Identification of a biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Data table header descriptions
VALUE Normalized signal Intensity calculated by Rosetta Resolver

Data table
PH_hs_0000002 151.246185
PH_hs_0000003 7.63040447
PH_hs_0000004 7.94024515
PH_hs_0000005 -1.79430127
PH_hs_0000006 860.818298
PH_hs_0000007 117.968369
PH_hs_0000008 31.2777214
PH_hs_0000009 4.69616699
PH_hs_0000010 1244.69238
PH_hs_0000011 128.734177
PH_hs_0000012 147.347748
PH_hs_0000013 214.805145
PH_hs_0000014 190.538147
PH_hs_0000015 432.676331
PH_hs_0000016 210.495117
PH_hs_0000017 0.06936729
PH_hs_0000018 3.14233923
PH_hs_0000019 2.1411376
PH_hs_0000020 191.853806
PH_hs_0000021 39.9093246

Total number of rows: 30968

Table truncated, full table size 755 Kbytes.

Supplementary file Size Download File type/resource
GSM840943_H015-0607008712.gpr.gz 2.6 Mb (ftp)(http) GPR
GSM840943_H016-0607008713.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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