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Sample GSM840942 Query DataSets for GSM840942
Status Public on Nov 30, 2011
Title HepG2_Oleic acid_rep3
Sample type RNA
 
Source name HepG2, Oleic acid, 7 days
Organism Homo sapiens
Characteristics cell line: HepG2
incubated fatty acid: Oleic acid
Treatment protocol Incubation for seven days with 100 µM fatty acid (oleic, elaidic or stearic acid)
Extracted molecule total RNA
Extraction protocol Total RNA Purification Kit (NORGEN BIOTEK CORPORATION) Distribubed by Phalanx Biotech
Label Cy5
Label protocol RNA was converted to double-stranded cDNA and amplified using an in vitro transcription reaction that included aminoallyl-UTP, and the aRNA product was conjugated with Cy5T NHS ester
 
Hybridization protocol Fragmented aRNA was hybridized at 42° C overnight using the HybBag mixing system with 1× OneArray Hybridization Buffer (Phalanx Biotech), 0.01 mg/mL sheared salmon sperm DNA (Promega) and 0.025 mg/mL labeled target. After hybridization, the arrays were washed according to the OneArray protocol
Scan protocol Raw intensity signals from each microarray were captured using a Molecular Dynamics™ Axon 4100A scanner, measured using GenePixPro™ Software, and stored in GPR format
Description OC
raw data file technical rep1: H013-0607008696.gpr
raw data file technical rep2: H014-0607008698.gpr
Data processing The data from all microarrays in each experimental set were passed to Rosetta Resolver (Rosetta Biosoftware) for analysis. Testing was performed by combining the technical replicates and performing statistical analyses using Rosetta Resolver's proprietary modeling techniques
 
Submission date Nov 30, 2011
Last update date Nov 30, 2011
Contact name Toke Krogager Hansen
E-mail(s) toke.krogager@gmail.com
Organization name Aarhus University
Department Molecular Biology
Lab Proteome analysis
Street address gustav wieds vej 10c
City Aarhus
ZIP/Postal code 8000
Country Denmark
 
Platform ID GPL6254
Series (1)
GSE34045 Identification of a biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Data table header descriptions
ID_REF
VALUE Normalized signal Intensity calculated by Rosetta Resolver

Data table
ID_REF VALUE
PH_hs_0000002 193.245178
PH_hs_0000003 9.10494518
PH_hs_0000004 27.1730766
PH_hs_0000005 6.06321335
PH_hs_0000006 818.865906
PH_hs_0000007 361.435547
PH_hs_0000008 39.6883278
PH_hs_0000009 18.9875374
PH_hs_0000010 1887.86255
PH_hs_0000011 145.217392
PH_hs_0000012 523.121216
PH_hs_0000013 434.238647
PH_hs_0000014 395.6763
PH_hs_0000015 1438.52869
PH_hs_0000016 608.326172
PH_hs_0000017 44.2650986
PH_hs_0000018 5.04255199
PH_hs_0000019 4.10289574
PH_hs_0000020 809.461182
PH_hs_0000021 128.643799

Total number of rows: 30968

Table truncated, full table size 754 Kbytes.




Supplementary file Size Download File type/resource
GSM840942_H013-0607008696.gpr.gz 2.6 Mb (ftp)(http) GPR
GSM840942_H014-0607008698.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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