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Sample GSM840939 Query DataSets for GSM840939
Status Public on Nov 30, 2011
Title HepG2_control_rep4
Sample type RNA
Source name HepG2, control, 7days
Organism Homo sapiens
Characteristics cell line: HepG2
incubated fatty acid: none
Treatment protocol Incubation for seven days with 100 µM fatty acid (oleic, elaidic or stearic acid)
Extracted molecule total RNA
Extraction protocol Total RNA Purification Kit (NORGEN BIOTEK CORPORATION) Distribubed by Phalanx Biotech
Label Cy5
Label protocol RNA was converted to double-stranded cDNA and amplified using an in vitro transcription reaction that included aminoallyl-UTP, and the aRNA product was conjugated with Cy5T NHS ester
Hybridization protocol Fragmented aRNA was hybridized at 42° C overnight using the HybBag mixing system with 1× OneArray Hybridization Buffer (Phalanx Biotech), 0.01 mg/mL sheared salmon sperm DNA (Promega) and 0.025 mg/mL labeled target. After hybridization, the arrays were washed according to the OneArray protocol
Scan protocol Raw intensity signals from each microarray were captured using a Molecular Dynamics™ Axon 4100A scanner, measured using GenePixPro™ Software, and stored in GPR format
Description CD
raw data file technical rep1: H007-0607008144.gpr
raw data file technical rep2: H008-0607008145.gpr
Data processing The data from all microarrays in each experimental set were passed to Rosetta Resolver (Rosetta Biosoftware) for analysis. Testing was performed by combining the technical replicates and performing statistical analyses using Rosetta Resolver's proprietary modeling techniques
Submission date Nov 30, 2011
Last update date Nov 30, 2011
Contact name Toke Krogager Hansen
Organization name Aarhus University
Department Molecular Biology
Lab Proteome analysis
Street address gustav wieds vej 10c
City Aarhus
ZIP/Postal code 8000
Country Denmark
Platform ID GPL6254
Series (1)
GSE34045 Identification of a biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Data table header descriptions
VALUE Normalized signal Intensity calculated by Rosetta Resolver

Data table
PH_hs_0000002 243.224945
PH_hs_0000003 29.9514961
PH_hs_0000004 81.4016953
PH_hs_0000005 1.12208271
PH_hs_0000006 751.234375
PH_hs_0000007 426.330627
PH_hs_0000008 68.3557968
PH_hs_0000009 85.0991592
PH_hs_0000010 1742.55737
PH_hs_0000011 184.937485
PH_hs_0000012 619.663452
PH_hs_0000013 650.939819
PH_hs_0000014 973.710938
PH_hs_0000015 3711.51172
PH_hs_0000016 1601.02954
PH_hs_0000017 38.9991341
PH_hs_0000018 25.8281822
PH_hs_0000019 10.4738989
PH_hs_0000020 2669.97559
PH_hs_0000021 138.634674

Total number of rows: 30968

Table truncated, full table size 754 Kbytes.

Supplementary file Size Download File type/resource
GSM840939_H007-0607008144.gpr.gz 2.7 Mb (ftp)(http) GPR
GSM840939_H008-0607008145.gpr.gz 2.7 Mb (ftp)(http) GPR
Processed data included within Sample table

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