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Sample GSM840938 Query DataSets for GSM840938
Status Public on Nov 30, 2011
Title HepG2_control_rep3
Sample type RNA
Source name HepG2, control, 7days
Organism Homo sapiens
Characteristics cell line: HepG2
incubated fatty acid: none
Treatment protocol Incubation for seven days with 100 µM fatty acid (oleic, elaidic or stearic acid)
Extracted molecule total RNA
Extraction protocol Total RNA Purification Kit (NORGEN BIOTEK CORPORATION) Distribubed by Phalanx Biotech
Label Cy5
Label protocol RNA was converted to double-stranded cDNA and amplified using an in vitro transcription reaction that included aminoallyl-UTP, and the aRNA product was conjugated with Cy5T NHS ester
Hybridization protocol Fragmented aRNA was hybridized at 42° C overnight using the HybBag mixing system with 1× OneArray Hybridization Buffer (Phalanx Biotech), 0.01 mg/mL sheared salmon sperm DNA (Promega) and 0.025 mg/mL labeled target. After hybridization, the arrays were washed according to the OneArray protocol
Scan protocol Raw intensity signals from each microarray were captured using a Molecular Dynamics™ Axon 4100A scanner, measured using GenePixPro™ Software, and stored in GPR format
Description CC
raw data file technical rep1: H005-0607008682.gpr
raw data file technical rep2: H006-0607008683.gpr
Data processing The data from all microarrays in each experimental set were passed to Rosetta Resolver (Rosetta Biosoftware) for analysis. Testing was performed by combining the technical replicates and performing statistical analyses using Rosetta Resolver's proprietary modeling techniques
Submission date Nov 30, 2011
Last update date Nov 30, 2011
Contact name Toke Krogager Hansen
Organization name Aarhus University
Department Molecular Biology
Lab Proteome analysis
Street address gustav wieds vej 10c
City Aarhus
ZIP/Postal code 8000
Country Denmark
Platform ID GPL6254
Series (1)
GSE34045 Identification of a biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Data table header descriptions
VALUE Normalized signal Intensity calculated by Rosetta Resolver

Data table
PH_hs_0000002 176.559784
PH_hs_0000003 8.6036787
PH_hs_0000004 17.5965729
PH_hs_0000005 1.53637123
PH_hs_0000006 707.898438
PH_hs_0000007 158.307693
PH_hs_0000008 31.8540974
PH_hs_0000009 13.6224918
PH_hs_0000010 1157.78882
PH_hs_0000011 100.028015
PH_hs_0000012 519.19104
PH_hs_0000013 365.779266
PH_hs_0000014 220.807266
PH_hs_0000015 1777.13086
PH_hs_0000016 559.874146
PH_hs_0000017 33.9230766
PH_hs_0000018 5.96112061
PH_hs_0000019 -0.532608688
PH_hs_0000020 1131.69104
PH_hs_0000021 51.171402

Total number of rows: 30968

Table truncated, full table size 754 Kbytes.

Supplementary file Size Download File type/resource
GSM840938_H005-0607008682.gpr.gz 2.6 Mb (ftp)(http) GPR
GSM840938_H006-0607008683.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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