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Sample GSM840936 Query DataSets for GSM840936
Status Public on Nov 30, 2011
Title HepG2_control_rep1
Sample type RNA
Source name HepG2, control, 7days
Organism Homo sapiens
Characteristics cell line: HepG2
incubated fatty acid: none
Treatment protocol Incubation for seven days with 100 µM fatty acid (oleic, elaidic or stearic acid)
Extracted molecule total RNA
Extraction protocol Total RNA Purification Kit (NORGEN BIOTEK CORPORATION) Distribubed by Phalanx Biotech
Label Cy5
Label protocol RNA was converted to double-stranded cDNA and amplified using an in vitro transcription reaction that included aminoallyl-UTP, and the aRNA product was conjugated with Cy5T NHS ester
Hybridization protocol Fragmented aRNA was hybridized at 42° C overnight using the HybBag mixing system with 1× OneArray Hybridization Buffer (Phalanx Biotech), 0.01 mg/mL sheared salmon sperm DNA (Promega) and 0.025 mg/mL labeled target. After hybridization, the arrays were washed according to the OneArray protocol
Scan protocol Raw intensity signals from each microarray were captured using a Molecular Dynamics™ Axon 4100A scanner, measured using GenePixPro™ Software, and stored in GPR format
Description CA
raw data file technical rep1: H001-0607008711.gpr
raw data file technical rep2: H002-0607008676.gpr
Data processing The data from all microarrays in each experimental set were passed to Rosetta Resolver (Rosetta Biosoftware) for analysis. Testing was performed by combining the technical replicates and performing statistical analyses using Rosetta Resolver's proprietary modeling techniques
Submission date Nov 30, 2011
Last update date Nov 30, 2011
Contact name Toke Krogager Hansen
Organization name Aarhus University
Department Molecular Biology
Lab Proteome analysis
Street address gustav wieds vej 10c
City Aarhus
ZIP/Postal code 8000
Country Denmark
Platform ID GPL6254
Series (1)
GSE34045 Identification of a biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Data table header descriptions
VALUE Normalized signal Intensity calculated by Rosetta Resolver

Data table
PH_hs_0000002 114.101227
PH_hs_0000003 10.0158749
PH_hs_0000004 13.1003895
PH_hs_0000005 6.25112629
PH_hs_0000006 612.359375
PH_hs_0000007 102.722534
PH_hs_0000008 5.48879337
PH_hs_0000009 20.7518616
PH_hs_0000010 871.808105
PH_hs_0000011 54.8832436
PH_hs_0000012 257.62146
PH_hs_0000013 151.396881
PH_hs_0000014 155.806671
PH_hs_0000015 652.256348
PH_hs_0000016 139.94313
PH_hs_0000017 14.7845583
PH_hs_0000018 18.3475838
PH_hs_0000019 13.4217415
PH_hs_0000020 176.441254
PH_hs_0000021 24.1600723

Total number of rows: 30968

Table truncated, full table size 755 Kbytes.

Supplementary file Size Download File type/resource
GSM840936_H001-0607008711.gpr.gz 2.6 Mb (ftp)(http) GPR
GSM840936_H002-0607008676.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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