|
| Status |
Public on Nov 30, 2011 |
| Title |
Cornea no DT infected |
| Sample type |
RNA |
| |
|
| Source name |
Whole corneas from bone marrow chimeric mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Cornea
infection: no DT infected
host strain: BALB/cJ
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from pooled whole corneas using RNeasy and Qiashredder kits (Qiagen, Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 2 - 2.5 ug total RNA using the Affymetrix 3’ IVT express kit (#901229, Affymetrix Inc., Santa Clara, CA)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The 2% trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Nov 29, 2011 |
| Last update date |
Nov 30, 2011 |
| Contact name |
Stephen Alan Kenneth Harvey |
| E-mail(s) |
stephenaharvey@cs.com
|
| Organization name |
University of Pittsburgh School of Medicine
|
| Department |
Ophthalmology
|
| Lab |
EEINS 1014
|
| Street address |
203 Lothrop St
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213-2588 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE33991 |
Early responding dendritic cells direct local natural killer response to control HSV-1 infection within the cornea |
|
