|
| Status |
Public on Dec 01, 2011 |
| Title |
WT Stat1 6hr IFNg BMM |
| Sample type |
SRA |
| |
|
| Source name |
bone marrow macrophages
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Ikbke+/+
cell type: Bone marrow macrophages
chip antibody: anti-STAT1α (santa cruz: sc-417)
chip antibody manufacturer: Santa Cruz
chip antibody catalog #: sc-417
treatment: IFNγ for 6 hour
genetic background: C58BL/6
|
| Growth protocol |
Bone marrow from Ikbke+/+ and Ikbke-/- mice was isolated, differentiated into macrophages with macrophage colony-stimulating factor (M-CSF), and either untreated or treated with IFNb or IFNg for six hours before crosslinking for chromatin isolation.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Standard Illumina library construction
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
Chromatin IP with STAT1 antibody
|
| Data processing |
STAT1 binding sites were identified with the program MACS, using the corresponding reverse cross-link sample as a control.
mm9
|
| |
|
| Submission date |
Nov 23, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Jason Gertz |
| E-mail(s) |
jgertz@hudsonalpha.com
|
| Organization name |
HudsonAlpha Institute
|
| Street address |
601 Genome Way
|
| City |
Huntsville |
| State/province |
AL |
| ZIP/Postal code |
35806 |
| Country |
USA |
| |
|
| Platform ID |
GPL11002 |
| Series (1) |
| GSE33913 |
IKK-epsilon regulates the balance between the type I and type II interferon responses |
|
| Relations |
| SRA |
SRX109331 |
| BioSample |
SAMN00760835 |
| Named Annotation |
GSM838683_MACS_SL914_peaks.bed.gz |
