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Sample GSM838681 Query DataSets for GSM838681
Status Public on Dec 01, 2011
Title WT Stat1 untreated BMM
Sample type SRA
Source name bone marrow macrophages
Organism Mus musculus
Characteristics genotype: Ikbke+/+
cell type: Bone marrow macrophages
chip antibody: anti-STAT1α (santa cruz: sc-417)
chip antibody manufacturer: Santa Cruz
chip antibody catalog #: sc-417
treatment: none
genetic background: C58BL/6
Growth protocol Bone marrow from Ikbke+/+ and Ikbke-/- mice was isolated, differentiated into macrophages with macrophage colony-stimulating factor (M-CSF), and either untreated or treated with IFNb or IFNg for six hours before crosslinking for chromatin isolation.
Extracted molecule genomic DNA
Extraction protocol Standard Illumina library construction
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
Description Chromatin IP with STAT1 antibody
Data processing STAT1 binding sites were identified with the program MACS, using the corresponding reverse cross-link sample as a control.
Submission date Nov 23, 2011
Last update date May 15, 2019
Contact name Jason Gertz
Organization name HudsonAlpha Institute
Street address 601 Genome Way
City Huntsville
State/province AL
ZIP/Postal code 35806
Country USA
Platform ID GPL11002
Series (1)
GSE33913 IKK-epsilon regulates the balance between the type I and type II interferon responses
SRA SRX109329
BioSample SAMN00760833
Named Annotation GSM838681_MACS_SL997_peaks.bed.gz

Supplementary file Size Download File type/resource
GSM838681_MACS_SL997_peaks.bed.gz 326.8 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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