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| Status |
Public on Jul 06, 2024 |
| Title |
dLN-BrQ3 mouse |
| Sample type |
SRA |
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| Source name |
Lymph node
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| Organism |
Mus musculus |
| Characteristics |
tissue: Lymph node
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| Extracted molecule |
total RNA |
| Extraction protocol |
lymph node samples from 4 different mouse were used for the genome-wide RNA-seq analysis. TRI Reagent (TR118, Molecular Research Center, Inc.) was used to extract total RNA according to the manufacturer’s protocol.
RNA samples with an RNA integrity number > 8 were used to prepare libraries, and paired-end sequencing was performed by CapitalBio Technology (China) following a standard protocol.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Sequencing reads were trimmed for adaptor sequence/low quality sequence using trim_galore version 0.6.2(parameter -q 20 --length 25 -e 0.1)
Trimmed sequence reads were mapped to mm19 using hisat2 version 2.1.0
Read count extraction were performed using HTSeq version 0.11.2 (parameter -a 10 -t exon)
Assembly: mm10
Supplementary files format and content: tab-delimited text files includes raw counts for each Sample
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| Submission date |
Jul 05, 2024 |
| Last update date |
Jul 06, 2024 |
| Contact name |
Yuanchen Ma |
| E-mail(s) |
maych25@mail.sysu.edu.cn
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| Phone |
18718410165
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| Organization name |
SYSU
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| Street address |
No74,Zhongshan Road2, Yuexiu District,Guangzhou,Guangdong China
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| City |
Guangzhou |
| State/province |
State |
| ZIP/Postal code |
520000 |
| Country |
China |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE271550 |
2-Bromo-1,4-Naphthalenedione Promoting CD8+ T Cell Expansion and Inhibits Th1/Th17 Differentiation to Mitigate Experimental Autoimmune Encephalomyelitis |
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| Relations |
| BioSample |
SAMN42255986 |
| SRA |
SRX25189994 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM8379575_dLN-BrQ3.count.txt.gz |
391.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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