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GEO accession:
Sample GSM835198
Query DataSets for GSM835198
Status
Public on Jun 18, 2012
Title
2C::tomato-, WT-1
Sample type
RNA
Source name
2C::tomato-, WT-1
Organism
Mus musculus
Characteristics
cell line: ES cells
Treatment protocol
Cells were treated with vehicle or 4OHT to delete Kdm1a, and 2C::tomato+ and - cells were collected using FACS.
Growth protocol
ES cells were passaged on gelatin in ES-KOSR media
Extracted molecule
total RNA
Extraction protocol
RNA was prepared using Qiagen Rneasy kit with on-column DNAse digestion
Label
biotin
Label protocol
Labeling was perfomred with 100ng total RNA using Whole Transcript Sense target labeling kit (Affymetrix)
Hybridization protocol
25ng/ul sense DNA was hybed at 45 degrees C, washed with Affy fluidics station 4500
Scan protocol
Affymetrix 300 7G scanner
Description
Replicate 1
Data processing
RMA using Expression Console
Submission date
Nov 16, 2011
Last update date
Jun 18, 2012
Contact name
Todd Macfarlan
E-mail(s)
macfarlan@salk.edu
Organization name
The Salk Institute
Street address
10010 North Torrey Pines
City
La Jolla
State/province
CA
ZIP/Postal code
92037
Country
USA
Platform ID
GPL6246
Series (2)
GSE33763
Expression data from 2C::tomato+ vs 2C::tomato - ES cells
GSE33923
2C::tomato ES cells, 2-cell embryos and wild type oocytes
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344614
7.476367
10344616
3.04633
10344620
4.787564
10344622
8.177641
10344624
11.21798
10344633
11.37978
10344637
9.434059
10344653
5.174681
10344658
8.145177
10344674
4.556478
10344679
5.86945
10344707
9.248866
10344713
12.48551
10344715
5.011968
10344717
4.073942
10344719
6.762661
10344721
3.180764
10344723
8.616148
10344725
7.883805
10344741
11.6166
Total number of rows:
35556
Table truncated, full table size
621 Kbytes
.
Supplementary file
Size
Download
File type/resource
GSM835198.CEL.gz
4.4 Mb
(ftp)
(http)
CEL
Processed data included within Sample table
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