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GEO accession:
Sample GSM835194
Query DataSets for GSM835194
Status
Public on Jun 18, 2012
Title
2C::tomato-, WT-3
Sample type
RNA
Source name
2C::tomato-, WT-3
Organism
Mus musculus
Characteristics
cell line: ES cells
Treatment protocol
Cells were treated with vehicle or 4OHT to delete Kdm1a, and 2C::tomato+ and - cells were collected using FACS.
Growth protocol
ES cells were passaged on gelatin in ES-KOSR media
Extracted molecule
total RNA
Extraction protocol
RNA was prepared using Qiagen Rneasy kit with on-column DNAse digestion
Label
biotin
Label protocol
Labeling was perfomred with 100ng total RNA using Whole Transcript Sense target labeling kit (Affymetrix)
Hybridization protocol
25ng/ul sense DNA was hybed at 45 degrees C, washed with Affy fluidics station 4500
Scan protocol
Affymetrix 300 7G scanner
Description
Replicate 3
Data processing
RMA using Expression Console
Submission date
Nov 16, 2011
Last update date
Jun 18, 2012
Contact name
Todd Macfarlan
E-mail(s)
macfarlan@salk.edu
Organization name
The Salk Institute
Street address
10010 North Torrey Pines
City
La Jolla
State/province
CA
ZIP/Postal code
92037
Country
USA
Platform ID
GPL6246
Series (2)
GSE33763
Expression data from 2C::tomato+ vs 2C::tomato - ES cells
GSE33923
2C::tomato ES cells, 2-cell embryos and wild type oocytes
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344614
7.375375
10344616
3.113301
10344620
4.919351
10344622
8.271551
10344624
11.1052
10344633
11.26098
10344637
9.462919
10344653
4.450692
10344658
7.996373
10344674
4.482315
10344679
5.772194
10344707
9.084088
10344713
12.70787
10344715
4.686623
10344717
4.179162
10344719
6.676901
10344721
3.246166
10344723
8.441009
10344725
7.477035
10344741
11.84415
Total number of rows:
35556
Table truncated, full table size
621 Kbytes
.
Supplementary file
Size
Download
File type/resource
GSM835194.CEL.gz
4.5 Mb
(ftp)
(http)
CEL
Processed data included within Sample table
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