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GEO accession:
Sample GSM835190
Query DataSets for GSM835190
Status
Public on Jun 18, 2012
Title
2C::tomato-, WT-2
Sample type
RNA
Source name
2C::tomato-, WT-2
Organism
Mus musculus
Characteristics
cell line: ES cells
Treatment protocol
Cells were treated with vehicle or 4OHT to delete Kdm1a, and 2C::tomato+ and - cells were collected using FACS.
Growth protocol
ES cells were passaged on gelatin in ES-KOSR media
Extracted molecule
total RNA
Extraction protocol
RNA was prepared using Qiagen Rneasy kit with on-column DNAse digestion
Label
biotin
Label protocol
Labeling was perfomred with 100ng total RNA using Whole Transcript Sense target labeling kit (Affymetrix)
Hybridization protocol
25ng/ul sense DNA was hybed at 45 degrees C, washed with Affy fluidics station 4500
Scan protocol
Affymetrix 300 7G scanner
Description
Replicate 2
Data processing
RMA using Expression Console
Submission date
Nov 16, 2011
Last update date
Jun 18, 2012
Contact name
Todd Macfarlan
E-mail(s)
macfarlan@salk.edu
Organization name
The Salk Institute
Street address
10010 North Torrey Pines
City
La Jolla
State/province
CA
ZIP/Postal code
92037
Country
USA
Platform ID
GPL6246
Series (2)
GSE33763
Expression data from 2C::tomato+ vs 2C::tomato - ES cells
GSE33923
2C::tomato ES cells, 2-cell embryos and wild type oocytes
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344614
7.219543
10344616
3.208291
10344620
4.661519
10344622
8.089765
10344624
11.3789
10344633
10.9384
10344637
9.377947
10344653
4.421629
10344658
8.171775
10344674
4.317645
10344679
5.649604
10344707
9.206138
10344713
12.58232
10344715
4.655172
10344717
4.078981
10344719
6.482858
10344721
3.230656
10344723
7.759019
10344725
7.41876
10344741
11.81848
Total number of rows:
35556
Table truncated, full table size
621 Kbytes
.
Supplementary file
Size
Download
File type/resource
GSM835190.CEL.gz
4.7 Mb
(ftp)
(http)
CEL
Processed data included within Sample table
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