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| Status |
Public on Oct 09, 2024 |
| Title |
M1 biol rep 1 |
| Sample type |
SRA |
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| Source name |
HBZY-1
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| Organism |
Rattus norvegicus |
| Characteristics |
group: LPS-induced
cell line: HBZY-1
cell type: glomerular mesangial cells
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using TRIzol ® Reagent ( Invitrogen ).
RNA libraries were prepared for sequencing using standard lllumina protocols.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
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| Data processing |
Fastp v 0.19.5 (https://github.com/OpenGene/fastp)was used for sequencing data quality control.
Hisat2 v 2.1.0 was used to align the quality-controlled raw data, cleandata ( reads ), with the reference genome to obtain mappeddata ( reads ) for subsequent transcript assembly, expression calculation, etc.
Quantitative analysis of gene and transcript expression levels was performed using software RSEM v 1.3.3(http://deweylab.biostat.wisc.edu/rsem/), respectively.
Assembly: Rattus _ norvegicus, reference genome version Rnor _ 6.0, reference genome source http://asia.ensembl.org/Rattus_norvegicus/Info/Index
Supplementary files format and content: tab-delimited matrix file includes gene TPM values counts for each Sample
Supplementary files format and content: tab-delimited matrix file includes transcript TPM values counts for each Sample
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| Submission date |
Jun 24, 2024 |
| Last update date |
Oct 09, 2024 |
| Contact name |
Xi Sun |
| E-mail(s) |
20220935200@bucm.edu.cn
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| Phone |
18811305127
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| Organization name |
Beijing University of Chinese Medicine
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| Street address |
Gongchen Street, Fangshan District
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| City |
Beijing |
| ZIP/Postal code |
102488 |
| Country |
China |
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| Platform ID |
GPL24688 |
| Series (1) |
| GSE270526 |
The effect and mechanism of freeze-dried powder of Poecilobdella manillensis on improving inflammatory injury of rat glomerular mesangial cells through TXNIP / NLRP3 pathway. |
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| Relations |
| BioSample |
SAMN42017552 |
| SRA |
SRX25034935 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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