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Sample GSM8346177 Query DataSets for GSM8346177
Status Public on Oct 09, 2024
Title M1 biol rep 1
Sample type SRA
 
Source name HBZY-1
Organism Rattus norvegicus
Characteristics group: LPS-induced
cell line: HBZY-1
cell type: glomerular mesangial cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cells using TRIzol ® Reagent ( Invitrogen ).
RNA libraries were prepared for sequencing using standard lllumina protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing Fastp v 0.19.5 (https://github.com/OpenGene/fastp)was used for sequencing data quality control.
Hisat2 v 2.1.0 was used to align the quality-controlled raw data, cleandata ( reads ), with the reference genome to obtain mappeddata ( reads ) for subsequent transcript assembly, expression calculation, etc.
Quantitative analysis of gene and transcript expression levels was performed using software RSEM v 1.3.3(http://deweylab.biostat.wisc.edu/rsem/), respectively.
Assembly: Rattus _ norvegicus, reference genome version Rnor _ 6.0, reference genome source http://asia.ensembl.org/Rattus_norvegicus/Info/Index
Supplementary files format and content: tab-delimited matrix file includes gene TPM values counts for each Sample
Supplementary files format and content: tab-delimited matrix file includes transcript TPM values counts for each Sample
 
Submission date Jun 24, 2024
Last update date Oct 09, 2024
Contact name Xi Sun
E-mail(s) 20220935200@bucm.edu.cn
Phone 18811305127
Organization name Beijing University of Chinese Medicine
Street address Gongchen Street, Fangshan District
City Beijing
ZIP/Postal code 102488
Country China
 
Platform ID GPL24688
Series (1)
GSE270526 The effect and mechanism of freeze-dried powder of Poecilobdella manillensis on improving inflammatory injury of rat glomerular mesangial cells through TXNIP / NLRP3 pathway.
Relations
BioSample SAMN42017552
SRA SRX25034935

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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