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Sample GSM8337657 Query DataSets for GSM8337657
Status Public on Jun 19, 2024
Title NCHS-015 Single-cell RNAseq of osteosarcoma patient metastasis. Right upper lobe
Sample type SRA
 
Source name lung tumor
Organism Homo sapiens
Characteristics tissue: lung tumor
Extracted molecule polyA RNA
Extraction protocol Following dissociation into either nuclei or single cell suspension, single-cell 3’ RNA-Seq or 10x multiomics libraries were prepared using Chromium Single Cell system (10x Genomics) according to the manufacturer's instructions
Libraries were constructed per 10x protocols
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description S0218 Human patient lung tumor - 3' single cell RNAseq
Data processing BCL files were converted to fastq files using cellranger mkfastq
Fastq files were processed using cellranger count
Further processing and analyses performed are outlined on GitHub (https://github.com/kidcancerlab/epithelial)
Assembly: hg19
Supplementary files format and content: Tar files for each sample include the contents of the filtered_feature_bc_matrix folder output by Cellranger count (barcodes.tsv.gz features.tsv.gz matrix.mtx.gz)
 
Submission date Jun 18, 2024
Last update date Jun 19, 2024
Contact name Matt Cannon
E-mail(s) matthewvc1@gmail.com
Organization name Nationwide Children's Hospital
Department Center for Childhood Cancer
Street address 700 Children's Dr.
City Columbus
State/province OH
ZIP/Postal code 43205
Country USA
 
Platform ID GPL24676
Series (1)
GSE270231 Aberrant activation of wound healing programs within the metastatic niche facilitates lung colonization by osteosarcoma cells - human data
Relations
BioSample SAMN41126094
SRA SRX24573332

Supplementary file Size Download File type/resource
GSM8337657_S0218_10x_matrices.tar.gz 46.1 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA

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