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Sample GSM833355 Query DataSets for GSM833355
Status Public on Dec 13, 2011
Title High dosage of LECT2 vs control, 3.5 hours, biological replicate 2 of 2
Sample type RNA
 
Channel 1
Source name Mouse peritoneal macrophages exposed to 0 μg/ml of LECT2 for 3 hours
Organism Mus musculus
Characteristics tissue: mouse peritonaeum
treatment: exposed to 0 μg/ml of LECT2 for 3 hours
Treatment protocol Mouse peritoneal macrophages were plated at 1×105 cells/cm2 in complete culture medium and incubated with 0,0.5 or 5 μg/ml LECT2 for 3.5 or 48 hours
Growth protocol Mouse peritoneal macrophages were isolated from mouse peritonaeum. After washed with phosphate-buffered saline (PBS) twice, peritoneal macrophages were plated and cultivated in Dulbecco’s modified Eagle’s medium-low glucose supplemented with 20% FBS.
Extracted molecule total RNA
Extraction protocol Total RNA from different cultured cells was extracted using Trizol (Invitrogen) according to the manufacturer’s instructions.
Label Cy5
Label protocol 1μg of total RNA was used to synthesize the double strand cDNA. RNA was amplified by in vitro transcription using Ambion’s MessageAmp™ II aRNA Amplification Kits (Life Technologies, Austin, TX, USA). Then, aRNA was reverse transcribed into cDNA and further labeled with cy3-dCTP with Klenow enzyme.
 
Channel 2
Source name Mouse peritoneal macrophages exposed to 5 μg/ml of LECT2 for 3 hours
Organism Mus musculus
Characteristics tissue: mouse peritonaeum
treatment: exposed to 5 μg/ml of LECT2 for 3 hours
Treatment protocol Mouse peritoneal macrophages were plated at 1×105 cells/cm2 in complete culture medium and incubated with 0,0.5 or 5 μg/ml LECT2 for 3.5 or 48 hours
Growth protocol Mouse peritoneal macrophages were isolated from mouse peritonaeum. After washed with phosphate-buffered saline (PBS) twice, peritoneal macrophages were plated and cultivated in Dulbecco’s modified Eagle’s medium-low glucose supplemented with 20% FBS.
Extracted molecule total RNA
Extraction protocol Total RNA from different cultured cells was extracted using Trizol (Invitrogen) according to the manufacturer’s instructions.
Label Cy3
Label protocol 1μg of total RNA was used to synthesize the double strand cDNA. RNA was amplified by in vitro transcription using Ambion’s MessageAmp™ II aRNA Amplification Kits (Life Technologies, Austin, TX, USA). Then, aRNA was reverse transcribed into cDNA and further labeled with cy3-dCTP with Klenow enzyme.
 
 
Hybridization protocol According to the instructions of Agilent Gene Expression Hybridization Kit
Scan protocol Scanned on an Agilent G2505C scanner.Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
Description Biological replicate 2 of 2, 5 μg/ml of LECT2 for 3.5 hours vs 0 μg/ml of LECT2 for 3.5 hours
Data processing Agilent Feature Extraction Software (version 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Nov 15, 2011
Last update date Dec 13, 2011
Contact name wang liang
E-mail(s) wangliangkexue@163.com
Organization name BioChainBJ
Street address 7A North Yongchang Road BDA
City Beijing
ZIP/Postal code 100176
Country China
 
Platform ID GPL11202
Series (1)
GSE33721 Mouse peritoneal macrophages at 3.5 or 48 hours, 0,0.5 or 5 μg/ml Leukocyte cell-derived chemotaxin 2 (LECT2),gene expression array, two independent biological replicates

Data table header descriptions
ID_REF
VALUE Normalized log2 ratio (Cy5/Cy3) representing treatment/control.

Data table
ID_REF VALUE
A_51_P100034 -0.46084982
A_51_P100174 -0.26568842
A_51_P100208 -2.010169
A_51_P100289 -0.45546436
A_51_P100298 0.22268482
A_51_P100309 -1.3424082
A_51_P100327 -1.5072441
A_51_P100347 -0.8117483
A_51_P100519 -2.4327097
A_51_P100537 -1.9547086
A_51_P100573 0.78612614
A_51_P100624 -1.8085792
A_51_P100625 -1.6525294
A_51_P100768 0.50030047
A_51_P100776 0.2670651
A_51_P100787 -0.7106123
A_51_P100828 0.6940193
A_51_P100852 -2.3846893
A_51_P100991 -1.0200176
A_51_P100997 0.14977068

Total number of rows: 39428

Table truncated, full table size 947 Kbytes.




Supplementary file Size Download File type/resource
GSM833355_US10283824_252665510717_S01_GE2_107_Sep09_1_2.txt.gz 4.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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