NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM833351 Query DataSets for GSM833351
Status Public on Dec 13, 2011
Title High dosage of LECT2 vs control, 3.5 hours, biological replicate 1 of 2
Sample type RNA
 
Channel 1
Source name Mouse peritoneal macrophages exposed to 0 μg/ml of LECT2 for 3 hours
Organism Mus musculus
Characteristics tissue: mouse peritonaeum
treatment: exposed to 0 μg/ml of LECT2 for 3 hours
Treatment protocol Mouse peritoneal macrophages were plated at 1×105 cells/cm2 in complete culture medium and incubated with 0,0.5 or 5 μg/ml LECT2 for 3.5 or 48 hours
Growth protocol Mouse peritoneal macrophages were isolated from mouse peritonaeum. After washed with phosphate-buffered saline (PBS) twice, peritoneal macrophages were plated and cultivated in Dulbecco’s modified Eagle’s medium-low glucose supplemented with 20% FBS.
Extracted molecule total RNA
Extraction protocol Total RNA from different cultured cells was extracted using Trizol (Invitrogen) according to the manufacturer’s instructions.
Label Cy5
Label protocol 1μg of total RNA was used to synthesize the double strand cDNA. RNA was amplified by in vitro transcription using Ambion’s MessageAmp™ II aRNA Amplification Kits (Life Technologies, Austin, TX, USA). Then, aRNA was reverse transcribed into cDNA and further labeled with cy3-dCTP with Klenow enzyme.
 
Channel 2
Source name Mouse peritoneal macrophages exposed to 5 μg/ml of LECT2 for 3 hours
Organism Mus musculus
Characteristics tissue: mouse peritonaeum
treatment: exposed to 5 μg/ml of LECT2 for 3 hours
Treatment protocol Mouse peritoneal macrophages were plated at 1×105 cells/cm2 in complete culture medium and incubated with 0,0.5 or 5 μg/ml LECT2 for 3.5 or 48 hours
Growth protocol Mouse peritoneal macrophages were isolated from mouse peritonaeum. After washed with phosphate-buffered saline (PBS) twice, peritoneal macrophages were plated and cultivated in Dulbecco’s modified Eagle’s medium-low glucose supplemented with 20% FBS.
Extracted molecule total RNA
Extraction protocol Total RNA from different cultured cells was extracted using Trizol (Invitrogen) according to the manufacturer’s instructions.
Label Cy3
Label protocol 1μg of total RNA was used to synthesize the double strand cDNA. RNA was amplified by in vitro transcription using Ambion’s MessageAmp™ II aRNA Amplification Kits (Life Technologies, Austin, TX, USA). Then, aRNA was reverse transcribed into cDNA and further labeled with cy3-dCTP with Klenow enzyme.
 
 
Hybridization protocol According to the instructions of Agilent Gene Expression Hybridization Kit
Scan protocol Scanned on an Agilent G2505C scanner.Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
Description Biological replicate 1 of 2, 5 μg/ml of LECT2 for 3.5 hours vs 0 μg/ml of LECT2 for 3.5 hours
Data processing Agilent Feature Extraction Software (version 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Nov 15, 2011
Last update date Dec 13, 2011
Contact name wang liang
E-mail(s) wangliangkexue@163.com
Organization name BioChainBJ
Street address 7A North Yongchang Road BDA
City Beijing
ZIP/Postal code 100176
Country China
 
Platform ID GPL11202
Series (1)
GSE33721 Mouse peritoneal macrophages at 3.5 or 48 hours, 0,0.5 or 5 μg/ml Leukocyte cell-derived chemotaxin 2 (LECT2),gene expression array, two independent biological replicates

Data table header descriptions
ID_REF
VALUE Normalized log2 ratio (Cy5/Cy3) representing treatment/control.

Data table
ID_REF VALUE
A_51_P100034 0.09919835
A_51_P100174 -0.5659946
A_51_P100208 -0.3587846
A_51_P100289 0.4005642
A_51_P100298 -0.10075004
A_51_P100309 0.12272549
A_51_P100327 -2.5433235
A_51_P100347 -0.3383427
A_51_P100519 -0.34294742
A_51_P100537 -1.5922142
A_51_P100573 -0.3169765
A_51_P100624 -0.33420578
A_51_P100625 -1.2990489
A_51_P100768 -0.33852762
A_51_P100776 -0.27683738
A_51_P100787 -0.008079539
A_51_P100828 0.25600332
A_51_P100852 -3.1682653
A_51_P100991 -0.37273976
A_51_P100997 -0.562747

Total number of rows: 39428

Table truncated, full table size 949 Kbytes.




Supplementary file Size Download File type/resource
GSM833351_US10283824_252665510716_S02_GE2_107_Sep09_1_2.txt.gz 4.2 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap