|
| Status |
Public on Dec 06, 2011 |
| Title |
Tcf1-/- ETP thymocyte, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse DN1 stage thymocytes, Lin-CD44+CD25-kit+.
|
| Organism |
Mus musculus |
| Characteristics |
tissue: thymus
genotype: Lin-CD44+CD25-kit+
age: DN1 thymocyte, 10 weeks
genotype/variation: Tcf1-/-
background strain: C57Bl/6
|
| Treatment protocol |
Mice were sacrificed at 10 weeks of age
|
| Growth protocol |
Tcf1-/- and Tcf1-/+ strains were bred and housed under pathogen-free conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using Arcturus PicoPure RNA Isolation kit (Life Technologies, Foster City, CA) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Average yield from 1000 cells was 3 ng of total RNA. The RNA was amplified using Ovation Pico WTA System (NuGen, San Carlos, CA). Labeling, fragmentation and hybridization to Mouse Genome 430 2.0 Arrays were done according to the manufacturer's instructions (Affymetrix Inc., Santa Clara, CA).
|
| |
|
| Hybridization protocol |
5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse430_2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from mouse ETP stage thymocytes with ablation of Tcf1.
|
| Data processing |
The data were analyzed with affy bioconductor package.
|
| |
|
| Submission date |
Nov 07, 2011 |
| Last update date |
Dec 06, 2011 |
| Contact name |
Jiangwen Zhang |
| E-mail(s) |
jzhang@cgr.harvard.edu
|
| Organization name |
Harvard University
|
| Department |
FAS Center for Systems Biology
|
| Street address |
7 Divinity Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE33513 |
T cell factor 1 is a gatekeeper for T-cell specification in response to Notch signaling |
|
