|
Status |
Public on Nov 11, 2011 |
Title |
Normal breast tissue Replicate 7 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
methylated CpG-island recovery assay (MIRA) enriched, normal breast tissue,non-breast cancer patient 7
|
Organism |
Homo sapiens |
Characteristics |
tissue: normal breast patient: 7 age: 35 years sample type: methylated
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was isolated from fresh frozen tissues using standard phenol/chloroform method.
|
Label |
cy5
|
Label protocol |
The products of whole genome amplification from the total input DNA without MBD enrichment and from methylation-enriched DNA were purified by using DNA purification kit, respectively. The purified DNA fragments were then labeled with cy3-dCTP or cy5-dCTP with Klenow enzyme (Takara) according to the instruction of the manufacturer.
|
|
|
Channel 2 |
Source name |
input, normal breast tissue,non-breast cancer patient 7
|
Organism |
Homo sapiens |
Characteristics |
tissue: normal breast patient: 7 sample type: Input
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was isolated from fresh frozen tissues using standard phenol/chloroform method.
|
Label |
cy3
|
Label protocol |
The products of whole genome amplification from the total input DNA without MBD enrichment and from methylation-enriched DNA were purified by using DNA purification kit, respectively. The purified DNA fragments were then labeled with cy3-dCTP or cy5-dCTP with Klenow enzyme (Takara) according to the instruction of the manufacturer.
|
|
|
|
Hybridization protocol |
According to the instructions of Agilent Oligo aCGH Hybridization Kit
|
Scan protocol |
Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
Description |
Biological replicate 7 of 10,normal breast tissue,non-breast cancer patient
|
Data processing |
Agilent GeneSpring GX 11.5 was used for background subtraction and LOWESS normalization. Following global mean normalization, probes with an intensity <400 were discarded from further analysis.
|
|
|
Submission date |
Nov 03, 2011 |
Last update date |
Jan 20, 2012 |
Contact name |
wang liang |
E-mail(s) |
wangliangkexue@163.com
|
Organization name |
BioChainBJ
|
Street address |
7A North Yongchang Road BDA
|
City |
Beijing |
ZIP/Postal code |
100176 |
Country |
China |
|
|
Platform ID |
GPL14835 |
Series (2) |
GSE33445 |
DNA hypermethylation in breast cancer: breast cancer tissues vs. normal breast tissues |
GSE33450 |
DNA hypermethylation and gene expression in breast cancer: breast cancer tissues vs. normal breast tissues |
|