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Sample GSM82689 Query DataSets for GSM82689
Status Public on Sep 06, 2006
Title proband i, past exhaustive exercise
Sample type RNA
 
Source name white blood cells
Organism Homo sapiens
Characteristics male, healthy, no medicamentation
Extracted molecule total RNA
Extraction protocol 9ml blood were collected and erythrocyte lysis using Qiagen Erythrocyte lysis buffer was performed immediately. White blood cells were washed in icecold PBS two times. Cells were then lysed using Trizol (Invitrogen).Phase lock gel tubes (Eppendorf) were used to extract the RNA containing phase. Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 3 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description proband i, past exhaustive exercise, white blood cells, male, healthy, no medicamentation
Data processing GCOS 1.2 and GeneSpring 7.1 (Agilent)
 
Submission date Nov 10, 2005
Last update date Sep 06, 2006
Contact name Sandy Mosig
E-mail(s) sandy.mosig@mti.uni-jena.de, sandy.mosig@googlemail.com
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
 
Platform ID GPL571
Series (1)
GSE3606 Gene expression profiles in white blood cells in response to exercise

Data table header descriptions
ID_REF Affymetrix annotation numbers
VALUE GCOS 1.2 -calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 511.3 P 0.000754
AFFX-BioB-M_at 963.4 P 0.000044
AFFX-BioB-3_at 395.2 P 0.000581
AFFX-BioC-5_at 1459.4 P 0.000081
AFFX-BioC-3_at 1321.6 P 0.000044
AFFX-BioDn-5_at 1454.1 P 0.000044
AFFX-BioDn-3_at 6597.3 P 0.00006
AFFX-CreX-5_at 16676 P 0.000052
AFFX-CreX-3_at 17684.3 P 0.000044
AFFX-DapX-5_at 18.5 A 0.131361
AFFX-DapX-M_at 27.2 A 0.340661
AFFX-DapX-3_at 5 A 0.921998
AFFX-LysX-5_at 13.4 A 0.5
AFFX-LysX-M_at 10.7 A 0.58862
AFFX-LysX-3_at 18.5 A 0.116113
AFFX-PheX-5_at 2.2 A 0.876448
AFFX-PheX-M_at 1.4 A 0.824672
AFFX-PheX-3_at 34 A 0.368438
AFFX-ThrX-5_at 10.6 A 0.686277
AFFX-ThrX-M_at 1.4 A 0.897835

Total number of rows: 22277

Table truncated, full table size 591 Kbytes.




Supplementary data files not provided

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