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Sample GSM82688 Query DataSets for GSM82688
Status Public on Sep 06, 2006
Title proband k, past moderate exercise
Sample type RNA
Source name white blood cells
Organism Homo sapiens
Characteristics male, healthy, no medicamentation
Extracted molecule total RNA
Extraction protocol 9ml blood were collected and erythrocyte lysis using Qiagen Erythrocyte lysis buffer was performed immediately. White blood cells were washed in icecold PBS two times. Cells were then lysed using Trizol (Invitrogen).Phase lock gel tubes (Eppendorf) were used to extract the RNA containing phase. Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 3 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description proband k, past moderate exercise, white blood cells, male, healthy, no medicamentation
Data processing GCOS 1.2 and GeneSpring 7.1 (Agilent)
Submission date Nov 10, 2005
Last update date Sep 06, 2006
Contact name Sandy Mosig
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
Platform ID GPL571
Series (1)
GSE3606 Gene expression profiles in white blood cells in response to exercise

Data table header descriptions
ID_REF Affymetrix annotation numbers
VALUE GCOS 1.2 -calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 342.3 P 0.000509
AFFX-BioB-M_at 680.5 P 0.00011
AFFX-BioB-3_at 237.2 P 0.010317
AFFX-BioC-5_at 1052.6 P 0.00011
AFFX-BioC-3_at 885.8 P 0.000044
AFFX-BioDn-5_at 1029.1 P 0.000052
AFFX-BioDn-3_at 4840.1 P 0.000095
AFFX-CreX-5_at 12390.9 P 0.000052
AFFX-CreX-3_at 14241.8 P 0.000044
AFFX-DapX-5_at 5.5 A 0.48511
AFFX-DapX-M_at 46.3 A 0.156732
AFFX-DapX-3_at 21.5 A 0.659339
AFFX-LysX-5_at 30.1 A 0.287743
AFFX-LysX-M_at 8.4 A 0.814869
AFFX-LysX-3_at 14.4 A 0.275146
AFFX-PheX-5_at 7.3 A 0.760937
AFFX-PheX-M_at 1.9 A 0.941556
AFFX-PheX-3_at 54.5 A 0.216524
AFFX-ThrX-5_at 8.3 A 0.631562
AFFX-ThrX-M_at 8.8 A 0.617401

Total number of rows: 22277

Table truncated, full table size 592 Kbytes.

Supplementary data files not provided

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