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Sample GSM82681 Query DataSets for GSM82681
Status Public on Sep 06, 2006
Title proband a, past moderate exercise
Sample type RNA
Source name white blood cells
Organism Homo sapiens
Characteristics male, healthy, no medicamentation
Extracted molecule total RNA
Extraction protocol 9ml blood were collected and erythrocyte lysis using Qiagen Erythrocyte lysis buffer was performed immediately. White blood cells were washed in icecold PBS two times. Cells were then lysed using Trizol (Invitrogen).Phase lock gel tubes (Eppendorf) were used to extract the RNA containing phase. Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 3 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description proband a, past moderate exercise, white blood cells, male, healthy, no medicamentation
Data processing GCOS 1.2 and GeneSpring 7.1 (Agilent)
Submission date Nov 10, 2005
Last update date Sep 06, 2006
Contact name Sandy Mosig
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
Platform ID GPL571
Series (1)
GSE3606 Gene expression profiles in white blood cells in response to exercise

Data table header descriptions
ID_REF Affymetrix annotation numbers
VALUE GCOS 1.2 -calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 536.1 P 0.000446
AFFX-BioB-M_at 1043.2 P 0.000044
AFFX-BioB-3_at 500.8 P 0.000857
AFFX-BioC-5_at 1448.3 P 0.000095
AFFX-BioC-3_at 1380.6 P 0.00007
AFFX-BioDn-5_at 1520.1 P 0.000044
AFFX-BioDn-3_at 7451.2 P 0.00007
AFFX-CreX-5_at 16917.1 P 0.000052
AFFX-CreX-3_at 18504.3 P 0.000044
AFFX-DapX-5_at 29.3 A 0.139482
AFFX-DapX-M_at 24.5 A 0.544587
AFFX-DapX-3_at 5.5 A 0.945787
AFFX-LysX-5_at 21.1 M 0.058444
AFFX-LysX-M_at 7.9 A 0.574038
AFFX-LysX-3_at 10.8 A 0.340661
AFFX-PheX-5_at 1.3 A 0.960339
AFFX-PheX-M_at 1.4 A 0.973889
AFFX-PheX-3_at 8.7 A 0.672935
AFFX-ThrX-5_at 16.6 A 0.574038
AFFX-ThrX-M_at 1.5 A 0.645547

Total number of rows: 22277

Table truncated, full table size 591 Kbytes.

Supplementary data files not provided

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