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Sample GSM82680 Query DataSets for GSM82680
Status Public on Sep 06, 2006
Title proband a, past exhaustive exercise
Sample type RNA
Source name white blood cells
Organism Homo sapiens
Characteristics male, healthy, no medicamentation
Extracted molecule total RNA
Extraction protocol 9ml blood were collected and erythrocyte lysis using Qiagen Erythrocyte lysis buffer was performed immediately. White blood cells were washed in icecold PBS two times. Cells were then lysed using Trizol (Invitrogen).Phase lock gel tubes (Eppendorf) were used to extract the RNA containing phase. Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 3 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description proband a, past exhaustive exercise, white blood cells, male, healthy, no medicamentation
Data processing GCOS 1.2 and GeneSpring 7.1 (Agilent)
Submission date Nov 10, 2005
Last update date Sep 06, 2006
Contact name Sandy Mosig
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
Platform ID GPL571
Series (1)
GSE3606 Gene expression profiles in white blood cells in response to exercise

Data table header descriptions
ID_REF Affymetrix annotation numbers
VALUE GCOS 1.2 -calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 694.9 P 0.00034
AFFX-BioB-M_at 1447.5 P 0.000044
AFFX-BioB-3_at 564.3 P 0.00039
AFFX-BioC-5_at 1741.8 P 0.000081
AFFX-BioC-3_at 1398.3 P 0.000052
AFFX-BioDn-5_at 1744.8 P 0.000044
AFFX-BioDn-3_at 8083.8 P 0.00007
AFFX-CreX-5_at 18008 P 0.000052
AFFX-CreX-3_at 22061.9 P 0.000044
AFFX-DapX-5_at 26.4 A 0.275146
AFFX-DapX-M_at 34.4 A 0.275146
AFFX-DapX-3_at 6.5 A 0.9273
AFFX-LysX-5_at 23.9 A 0.340661
AFFX-LysX-M_at 35.6 A 0.544587
AFFX-LysX-3_at 29.8 A 0.227636
AFFX-PheX-5_at 9.2 A 0.699394
AFFX-PheX-M_at 2.3 A 0.937071
AFFX-PheX-3_at 28.9 A 0.440646
AFFX-ThrX-5_at 9.9 A 0.772364
AFFX-ThrX-M_at 16.2 A 0.5

Total number of rows: 22277

Table truncated, full table size 592 Kbytes.

Supplementary data files not provided

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