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Sample GSM82673 Query DataSets for GSM82673
Status Public on Sep 06, 2006
Title proband f, past moderate exercise
Sample type RNA
Source name white blood cells
Organism Homo sapiens
Characteristics male, healthy, no medicamentation
Extracted molecule total RNA
Extraction protocol 9ml blood were collected and erythrocyte lysis using Qiagen Erythrocyte lysis buffer was performed immediately. White blood cells were washed in icecold PBS two times. Cells were then lysed using Trizol (Invitrogen).Phase lock gel tubes (Eppendorf) were used to extract the RNA containing phase. Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 3 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description proband f, past moderate exercise, white blood cells, male, healthy, no medicamentation
Data processing GCOS 1.2 and GeneSpring 7.1 (Agilent)
Submission date Nov 10, 2005
Last update date Sep 06, 2006
Contact name Sandy Mosig
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
Platform ID GPL571
Series (1)
GSE3606 Gene expression profiles in white blood cells in response to exercise

Data table header descriptions
ID_REF Affymetrix annotation numbers
VALUE GCOS 1.2 -calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 440.5 P 0.000581
AFFX-BioB-M_at 1013.7 P 0.000052
AFFX-BioB-3_at 427.2 P 0.000754
AFFX-BioC-5_at 1336.8 P 0.000081
AFFX-BioC-3_at 1133.2 P 0.000044
AFFX-BioDn-5_at 1317.6 P 0.000044
AFFX-BioDn-3_at 7163.9 P 0.00007
AFFX-CreX-5_at 16041.6 P 0.000052
AFFX-CreX-3_at 19739.4 P 0.000044
AFFX-DapX-5_at 18.7 A 0.340648
AFFX-DapX-M_at 37.9 A 0.250796
AFFX-DapX-3_at 9.1 A 0.83416
AFFX-LysX-5_at 17.3 A 0.659352
AFFX-LysX-M_at 41 A 0.440641
AFFX-LysX-3_at 21 A 0.195266
AFFX-PheX-5_at 2.8 A 0.957038
AFFX-PheX-M_at 4.1 A 0.891021
AFFX-PheX-3_at 37.6 A 0.368438
AFFX-ThrX-5_at 47.1 A 0.574038
AFFX-ThrX-M_at 5.8 A 0.617401

Total number of rows: 22277

Table truncated, full table size 592 Kbytes.

Supplementary data files not provided

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