|
| Status |
Public on Jul 07, 2024 |
| Title |
PD-L1 3 |
| Sample type |
SRA |
| |
|
| Source name |
Breast tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Breast tumor
genotype: MMTV-PyMT
treatment: anti-PD-L1
|
| Treatment protocol |
FVB mice harboring PyMT tumors were treated with vehicle, twice weekly 5 mg/kg anti-PD-L1, daily 20 mg/kg sonidegib, or combination for 28 days
|
| Growth protocol |
Breast tumors were collected from 12 week old transgenic MMTV-PyMT mice and reimplanted into the 4th mammary fat pad of FVB mice
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was harvested using Qiazol (Qiagen).
Ribosomal (r)RNA-depleted stranded libraries
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
BO-7_2-2253226_S7
|
| Data processing |
Raw sequence reads were aligned using Gencode vM23 mouse genome annotation and Gencode Grcm38.p6 mouse reference genome using TopHat with default parameters.
Cufflinks were used to assemble and quantify transcripts from the mapped sequences.
Assembly: mm10
Supplementary files format and content: tab-delimited text file includes raw count for each sample
|
| |
|
| Submission date |
May 13, 2024 |
| Last update date |
Jul 07, 2024 |
| Contact name |
Wyatt Wofford |
| E-mail(s) |
woffordw@musc.edu
|
| Organization name |
MUSC
|
| Street address |
86 Jonathan Lucas St
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE267312 |
Alterations of Ceramide Synthesis Induce PD-L1 Internalization and Signaling to Regulate Tumor Metastasis and Immunotherapy Response |
|
| Relations |
| BioSample |
SAMN41381069 |
| SRA |
SRX24534131 |
