|
Status |
Public on May 21, 2024 |
Title |
Day 0, IP, ONH, Replicate 5 |
Sample type |
SRA |
|
|
Source name |
Optic Nerve Head
|
Organism |
Mus musculus |
Characteristics |
tissue: Optic Nerve Head batch: 1
|
Treatment protocol |
RNA was isolated from untreated animals
|
Growth protocol |
RNA was directly extracted from mouse retinal astrocytes from freshly ennucleated eyes
|
Extracted molecule |
polyA RNA |
Extraction protocol |
mRNA extraction was performed with oligo-dT beads and capturing poly(A) tails cDNA library preparation was done with the Smart-Seq HT Ultra-low input RNA kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Description |
X0_IP_ONH_5
|
Data processing |
Transcripts were quantified by a quasi-alignment approach using Salmon (version 1.6.0) Assembly: mm10 Supplementary files format and content: Cobined counts data containing data from each sample
|
|
|
Submission date |
Apr 26, 2024 |
Last update date |
May 21, 2024 |
Contact name |
William Jacob Gammerdinger |
Organization name |
Harvard University
|
Street address |
Massachusetts Hall
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02138 |
Country |
USA |
|
|
Platform ID |
GPL21103 |
Series (1) |
GSE265994 |
Transcriptional profiling of retinal astrocytes identifies a specific marker and points to functional specialization |
|
Relations |
BioSample |
SAMN41097678 |
SRA |
SRX24381905 |