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Sample GSM823856 Query DataSets for GSM823856
Status Public on Dec 06, 2011
Title PRB28959
Sample type RNA
 
Channel 1
Source name Mouse prostate stem cells
Organism Mus musculus
Characteristics strain: BL6
tissue type: prostate
facs: CD44+CD133+Sca-1+CD117+
cell type: stem cell
Treatment protocol The isolated primary cells were sorted by immunoflurescent staining and FACS.
Growth protocol Cells were isolated directly from the prostate tissue of BL6 mice
Extracted molecule total RNA
Extraction protocol PicoPure RNA isolation kit. The samples were amplifed using Message Amp II aRNA Amplification kit before labeling.
Label Cy5
Label protocol Quick Amp Labeling kit
 
Channel 2
Source name Universal Mouse Reference (Stratagene)
Organism Mus musculus
Characteristics reference: Universal Mouse Reference (Stratagene)
Extracted molecule total RNA
Extraction protocol PicoPure RNA isolation kit. The samples were amplifed using Message Amp II aRNA Amplification kit before labeling.
Label Cy3
Label protocol Quick Amp Labeling kit
 
 
Hybridization protocol Cyanine-5 dye was then incorporated through an IVT reaction using the Quick Amp Labeling kit (Agilent Technologies). 750 ng of each Cyanine-5 labeled test sample was pooled with Cyanine-3 labeled Universal Mouse Reference (Stratagene) and hybridized onto Agilent’s Whole Mouse Genome 4x44K arrays as described in the manufacturer’s protocol.
Scan protocol GeneChip scanner 3000.
Description 26926_CD44+CD133+Sca-1+CD117+
Data processing Agilent's Feature Extraction software 10.7
 
Submission date Oct 28, 2011
Last update date Dec 06, 2011
Contact name Yuting Sun
Organization name Genentech
Department Research Oncology
Street address 1 DNA way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platform ID GPL7202
Series (1)
GSE33317 Gene expression profiling of mouse prostate stem cells

Data table header descriptions
ID_REF
VALUE log2 Normalized ratio against Cyaine-3 labeled universal mouse reference
RATIO Normalized ratio against Cyaine-3 labeled universal mouse reference

Data table
ID_REF VALUE RATIO
A_51_P195244 1.5496 2.927269459
A_51_P397375 2.6478 6.267055988
A_52_P221804 -3.6211 0.081269979
A_52_P155692 -3.6512 0.079595342
A_51_P293015 1.4953 2.81923461
A_52_P348648 0.0000 1
A_51_P183723 -0.9782 0.507627666
A_51_P446510 3.5542 11.7470932
A_51_P520397 0.2567 1.194715619
A_51_P325217 0.0000 1
A_51_P493037 -5.4056 0.023592122
A_52_P109232 0.0000 1
A_51_P379873 0.0000 1
A_52_P235102 -1.3006 0.405970782
A_51_P279712 2.2545 4.771582603
A_52_P564046 0.0000 1
A_51_P258098 2.7080 6.534222603
A_52_P573543 -0.0212 0.985424578
A_52_P339054 -2.1815 0.220441505
A_52_P514306 -3.9235 0.065902285

Total number of rows: 41267

Table truncated, full table size 1245 Kbytes.




Supplementary file Size Download File type/resource
GSM823856_PRB28959.txt.gz 14.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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