|
| Status |
Public on Jul 02, 2024 |
| Title |
SH01, WT1-M |
| Sample type |
SRA |
| |
|
| Source name |
bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone marrow
genotype: WT
treatment: M
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed using TRIzol reagent (Invitrogen) and RNA was isolated using a phenol/chloroform and isopropanol precipitation with high salt.
Library preparation conducted by the Washington University in St. Louis Genome Technology Access Center (GTAC).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Base calls and demultiplexing were performed using Illumina bcl2fast
Reads were aligned to Ensemble release 76 primary assembly with STAR 2.5.1a
Counts were obtained using Subread:featureCount and Salmon 0.8.2
Sequencing QC was performed using RSeQC 2.6.2
Assembly: GENCODE M3 (GRCm38.p3)
Supplementary files format and content: raw-delimited text file includes raw counts for each sample
|
| |
|
| Submission date |
Apr 24, 2024 |
| Last update date |
Jul 02, 2024 |
| Contact name |
Christina Stallings |
| E-mail(s) |
stallings@wustl.edu
|
| Organization name |
Washington University in Saint Louis
|
| Street address |
425 South Euclid ave
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE265840 |
BHLHE40 regulates myeloid cell polarization through IL-10-dependent and independent mechanisms |
|
| Relations |
| BioSample |
SAMN41076707 |
| SRA |
SRX24362074 |
