|
| Status |
Public on Apr 24, 2024 |
| Title |
Offspring of control diet-fed dam, repeat 1 |
| Sample type |
SRA |
| |
|
| Source name |
Heart
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Heart
treatment: Offspring of control diet-fed dam
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Hearts were excised and continiousy perfused with enzymatic buffer solution containing 2 mg/mL collagenase IV (LS004188, Worthington Biochem) and 1 mg/mL dispase II (04942078001, Roche). Single cell suspension were then stained with antibodies and with Sytox™ Green (S34860, Invitrogen) and Vybrant™ DyeCycle™ (V10273, Invitrogen) and verapamil and sorted for live, metabolically active and nucleated cells using FACSAria™ III cell sorter (B.D.)
Libraries were prepared using according to the 10x Genomics chemistry 3` v2 kit and sequenced in an Illumina HiSeq instrument. Analysis was performed using 10X Genomics pipeline.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Cell ranger version 3.1.0
Assembly: mm10
Supplementary files format and content: 3 processed files generated by cell ranger, barcodes.tsv.gz, features.tsv.gz, and matrix.mtx.gz are provided.
|
| |
|
| Submission date |
Apr 23, 2024 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Francine Coelho Marques |
| E-mail(s) |
Francine.Marques@monash.edu
|
| Organization name |
Monash University
|
| Department |
School of Biological Sciences
|
| Lab |
The Hypertension Research Laboratory
|
| Street address |
25 Rainforest Walk
|
| City |
Clayton |
| ZIP/Postal code |
3800 |
| Country |
Australia |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE264691 |
single-cell RNA-seq of cardiac tissue from offsprings of dams with different fibre intake levels |
|
| Relations |
| BioSample |
SAMN41042868 |
| SRA |
SRX24330199 |
