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Sample GSM820732 Query DataSets for GSM820732
Status Public on Oct 25, 2011
Title Nrl-KO_rep2
Sample type SRA
 
Source name Retina
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: Nrl-/-
age: post natal day 21
tissue: Retina
Treatment protocol Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent.
Growth protocol Normal mouse growth protocol.
Extracted molecule total RNA
Extraction protocol Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer IIx
 
Description RNA-seq of Nrl-/- mouse retina
Data processing Illumina Casava1.7 software, BWA, Partek
 
Submission date Oct 21, 2011
Last update date May 15, 2019
Contact name HARSHA KARUR RAJASIMHA
E-mail(s) rajasimhah@nei.nih.gov
Phone 3014025734
Organization name NEI
Department ITMB
Lab NNRL
Street address 6 CENTER DR RM 303D
City BETHESDA
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL11002
Series (1)
GSE33141 Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Nrl-/- Retinal Transcriptomes
Relations
SRA SRX103290
BioSample SAMN00744415

Supplementary file Size Download File type/resource
GSM820732_R22011-023-KO2-bwa.bam 2.6 Gb (ftp)(http) BAM
GSM820732_R22011-023-KO2-tophat.bam 1.9 Gb (ftp)(http) BAM
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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