|
| Status |
Public on Oct 25, 2011 |
| Title |
Nrl-KO_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Retina
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Nrl-/-
age: post natal day 21
tissue: Retina
|
| Treatment protocol |
Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent.
|
| Growth protocol |
Normal mouse growth protocol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
RNA-seq of Nrl-/- mouse retina
|
| Data processing |
Illumina Casava1.7 software, BWA, Partek
|
| |
|
| Submission date |
Oct 21, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
HARSHA KARUR RAJASIMHA |
| E-mail(s) |
rajasimhah@nei.nih.gov
|
| Phone |
3014025734
|
| Organization name |
NEI
|
| Department |
ITMB
|
| Lab |
NNRL
|
| Street address |
6 CENTER DR RM 303D
|
| City |
BETHESDA |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL11002 |
| Series (1) |
| GSE33141 |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Nrl-/- Retinal Transcriptomes |
|
| Relations |
| SRA |
SRX103290 |
| BioSample |
SAMN00744415 |
