|
Status |
Public on Oct 25, 2011 |
Title |
wild-type_rep3 |
Sample type |
SRA |
|
|
Source name |
Retina
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 genotype: wild type age: post natal day 21 tissue: Retina
|
Treatment protocol |
Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent.
|
Growth protocol |
Normal mouse growth protocol.
|
Extracted molecule |
total RNA |
Extraction protocol |
Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina Genome Analyzer IIx |
|
|
Description |
RNA-seq of wild type mouse retina
|
Data processing |
Illumina Casava1.7 software, BWA, Partek
|
|
|
Submission date |
Oct 21, 2011 |
Last update date |
May 15, 2019 |
Contact name |
HARSHA KARUR RAJASIMHA |
E-mail(s) |
rajasimhah@nei.nih.gov
|
Phone |
3014025734
|
Organization name |
NEI
|
Department |
ITMB
|
Lab |
NNRL
|
Street address |
6 CENTER DR RM 303D
|
City |
BETHESDA |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL11002 |
Series (1) |
GSE33141 |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Nrl-/- Retinal Transcriptomes |
|
Relations |
SRA |
SRX103288 |
BioSample |
SAMN00744413 |