|
| Status |
Public on Jun 24, 2024 |
| Title |
MNF-70NO-2# |
| Sample type |
SRA |
| |
|
| Source name |
Cell
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Cell
cell line: iPSC
cell type: cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using FreeZol reagent (Vazyme),1 µg of RNA was used for sequencing libraries construction
Liarbries were constructed by VAHTS mRNA-seq v3 Library Prep Kit for Illumina (NR611, Vazyme)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The RNA-seq reads were trimmed using Trim Galore (v0.6.4) and then mapped to the mm10 reference genome with HISAT2 (v2.2.1) , and StringTie (v2.2.1) was used to quantify the transcription level of each gene in each sample into FPKM (Fragments per kilobase of exon model per million mapped reads).
Assembly: mm10
Supplementary files format and content: expression matrix(FPKM)
|
| |
|
| Submission date |
Apr 09, 2024 |
| Last update date |
Jun 24, 2024 |
| Contact name |
Chengchen Zhao |
| E-mail(s) |
zhaochengchen@westlake.edu.cn
|
| Organization name |
Westlake University
|
| Lab |
Laboratory of Cell Fate Control
|
| Street address |
Dunyu Road No.600, Xihu District
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310030 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE243517 |
Rational cell fate engineering through chromatin dynamics |
| GSE263598 |
Rational cell fate engineering through chromatin dynamics [RNA-Seq II] |
|
| Relations |
| BioSample |
SAMN40904896 |
| SRA |
SRX24201535 |
