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Sample GSM818947 Query DataSets for GSM818947
Status Public on Nov 01, 2011
Title H3K4me3 ChIP-Seq in GFP-transfected C2C12 cells
Sample type SRA
Source name C2C12 muscle progenitor cells
Organism Mus musculus
Characteristics cell type: GFP-transfected C2C12 cells
differentiation specificity: None
chip antibody: H3K4me3 (Abcam)
Treatment protocol Formaldehyde 1% crosslink 9min at 37°C. Cells were mechanically dissociated from the surface before lysis.
Growth protocol Mouse ES-cells were differentiated with B27 and N2 supplement (GIBCO) as monolayers on gelatinized cellbinding surface (Corning) with 0.5uM retinoic acid (all-trans, Sigma) at day 0 and day 2. C2C12 cells were cultured with serum unless for 3h during lipofectamine transfection.
Extracted molecule genomic DNA
Extraction protocol Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Prep Kit (1003473).
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
Description Chromatin IP against H3K4me3
Data processing Reads were aligned by Bowtie, with options -n 2 -m 1, against genome build mm9.
Submission date Oct 18, 2011
Last update date May 15, 2019
Contact name Rickard Sandberg
Organization name Karolinska Institutet
Department Department of Cell and Molecular Biology
Street address Berzelius vag 35
City Stockholm
ZIP/Postal code 17177
Country Sweden
Platform ID GPL13112
Series (2)
GSE33024 Sequentially acting Sox transcription factors in neural lineage development
GSE33059 Sequentially acting Sox transcription factors in neural lineage development [ChIP-seq]
SRA SRX101695
BioSample SAMN00739998

Supplementary file Size Download File type/resource
GSM818947_cz2_H3K27me3_C2C12+Sox3_chipseq.bed.gz 126.9 Mb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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