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Sample GSM818526 Query DataSets for GSM818526
Status Public on Sep 01, 2017
Title Heart MR-Cardio no treatment sample 2 (mouse n° 211)
Sample type RNA
 
Channel 1
Source name Heart MR-Cardio no treatment
Organism Mus musculus
Characteristics Sex: male
genotype: Transgenic (cardiomyocytes targeted mineralocorticoid receptor overexpression)
treatment: no treatment
treatment duration: 7 days
tissue: Heart
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from ventricle samples with the RNeasy Mini Kit (Qiagen S.A) according to the manufacturer's instructions and its quality was assessed with Bioanalyzer 2100 (Agilent technologies)
Label Cy5
Label protocol Labelling of the RNAs weas performed according to Agilent's instruction. Briefly, 100 ng of total RNA + spikes were primed with 1,8µl of T7 promoter primer mix at 70°C for 10 min, then reversed transcribed at 40°C for 2 h in presence of a mix containing first strand buffer, 20 mM DTT, 2 mM DNTP mix and AffinityScript rnase block mix. Reaction was stopped by heating samples at 70° foc 15 min. New synthetized cDNA was then labelled and amplified at 40° for 2h in presence of a mix containing transcription buffer, NTP mix, 20mM DTT, T7 RNA polymerase and CY3-CTP or CY5-CTP.
 
Channel 2
Source name Total RNA pooled from several tissues (liver, brain, heart kidney, spleen), labeled with Cyanine-3, used as reference
Organism Mus musculus
Characteristics tissues: liver, brain, heart kidney, spleen
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from ventricle samples with the RNeasy Mini Kit (Qiagen S.A) according to the manufacturer's instructions and its quality was assessed with Bioanalyzer 2100 (Agilent technologies)
Label Cy3
Label protocol Labelling of the RNAs weas performed according to Agilent's instruction. Briefly, 100 ng of total RNA + spikes were primed with 1,8µl of T7 promoter primer mix at 70°C for 10 min, then reversed transcribed at 40°C for 2 h in presence of a mix containing first strand buffer, 20 mM DTT, 2 mM DNTP mix and AffinityScript rnase block mix. Reaction was stopped by heating samples at 70° foc 15 min. New synthetized cDNA was then labelled and amplified at 40° for 2h in presence of a mix containing transcription buffer, NTP mix, 20mM DTT, T7 RNA polymerase and CY3-CTP or CY5-CTP.
 
 
Hybridization protocol 825 ng of Cy5-labelled samples and 825 ng of Cy3-labelled references were incubated at 60° for 30 min in fragmentation buffer. Hybridization buffer was then added, and sample/reference couples (1 couple per microarray) were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
Scan protocol Scanned on an G2505 Agilent scanner.
Images were quantified using Agilent Feature Extraction Software (version v 10.7.3.1).
Description Hearts were rinsed in cold PBS, frozen in liquid nitrogen, and kept at –80°C.
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.Data were processed using genespring software v11.0.
 
Submission date Oct 17, 2011
Last update date Sep 01, 2017
Contact name Smail Messaoudi
E-mail(s) smail.messaoudi@gmail.com
Phone +1 613 562 5800
Organization name University of Ottawa
Department BMI
Street address 451 smyth road
City Ottawa
State/province Ontario
ZIP/Postal code K1H 8M5
Country Canada
 
Platform ID GPL11202
Series (2)
GSE33022 Cardiac aldosterone and corticosterone regulated genes in mice with cardiomyocytes targeted mineralocorticoid receptor overexpression (MR-Cardio mice) and their controls (Ctrl mice)
GSE34049 Cardiac aldosterone regulated genes in mice with cardiomyocytes targeted mineralocorticoid receptor overexpression (MR-Cardio mice) and their controls (Ctrl mice)

Data table header descriptions
ID_REF
VALUE normalized log ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
GE_BrightCorner -0.63925564
DarkCorner -0.27118546
A_55_P1989846 -0.1271801
A_55_P1991598 -0.3181463
A_55_P2022211 -1.019867
A_55_P1980764 1.463037
A_55_P1964375 0.94750494
A_51_P128876 -1.2495775
A_55_P2121042 -0.29922187
A_52_P219230 -1.3044648
A_51_P207591 -0.8050065
A_55_P2131920 2.5514154
A_55_P2404223 0.97150093
A_55_P2101944 0.3445301
A_52_P358860 -1.7800751
A_51_P119031 0.8137922
A_51_P309854 -0.47754574
A_51_P343900 0.24336244
A_51_P234359 -1.5571369
A_51_P487813 -1.29179

Total number of rows: 39485

Table truncated, full table size 941 Kbytes.




Supplementary file Size Download File type/resource
GSM818526_galdo_252665510753_S01_GE2_107_Sep09_1_3.txt.gz 15.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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