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Sample GSM818520 Query DataSets for GSM818520
Status Public on Sep 01, 2017
Title Heart Ctrl + corticosterone treatment sample 1 (mouse n° 247)
Sample type RNA
 
Channel 1
Source name Heart Ctrl + corticosterone treatment
Organism Mus musculus
Characteristics Sex: male
genotype: Ctrl (non-transgenic)
treatment: Corticosterone
treatment duration: 7 days
tissue: Heart
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from ventricle samples with the RNeasy Mini Kit (Qiagen S.A) according to the manufacturer's instructions and its quality was assessed with Bioanalyzer 2100 (Agilent technologies)
Label Cy5
Label protocol Labelling of the RNAs weas performed according to Agilent's instruction. Briefly, 100 ng of total RNA + spikes were primed with 1,8µl of T7 promoter primer mix at 70°C for 10 min, then reversed transcribed at 40°C for 2 h in presence of a mix containing first strand buffer, 20 mM DTT, 2 mM DNTP mix and AffinityScript rnase block mix. Reaction was stopped by heating samples at 70° foc 15 min. New synthetized cDNA was then labelled and amplified at 40° for 2h in presence of a mix containing transcription buffer, NTP mix, 20mM DTT, T7 RNA polymerase and CY3-CTP or CY5-CTP.
 
Channel 2
Source name Total RNA pooled from several tissues (liver, brain, heart kidney, spleen), labeled with Cyanine-3, used as reference
Organism Mus musculus
Characteristics tissues: liver, brain, heart kidney, spleen
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from ventricle samples with the RNeasy Mini Kit (Qiagen S.A) according to the manufacturer's instructions and its quality was assessed with Bioanalyzer 2100 (Agilent technologies)
Label Cy3
Label protocol Labelling of the RNAs weas performed according to Agilent's instruction. Briefly, 100 ng of total RNA + spikes were primed with 1,8µl of T7 promoter primer mix at 70°C for 10 min, then reversed transcribed at 40°C for 2 h in presence of a mix containing first strand buffer, 20 mM DTT, 2 mM DNTP mix and AffinityScript rnase block mix. Reaction was stopped by heating samples at 70° foc 15 min. New synthetized cDNA was then labelled and amplified at 40° for 2h in presence of a mix containing transcription buffer, NTP mix, 20mM DTT, T7 RNA polymerase and CY3-CTP or CY5-CTP.
 
 
Hybridization protocol 825 ng of Cy5-labelled samples and 825 ng of Cy3-labelled references were incubated at 60° for 30 min in fragmentation buffer. Hybridization buffer was then added, and sample/reference couples (1 couple per microarray) were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
Scan protocol Scanned on an G2505 Agilent scanner.
Images were quantified using Agilent Feature Extraction Software (version v 10.7.3.1).
Description Hearts were rinsed in cold PBS, frozen in liquid nitrogen, and kept at –80°C.
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.Data were processed using genespring software v11.0.
 
Submission date Oct 17, 2011
Last update date Sep 01, 2017
Contact name Smail Messaoudi
E-mail(s) smail.messaoudi@gmail.com
Phone +1 613 562 5800
Organization name University of Ottawa
Department BMI
Street address 451 smyth road
City Ottawa
State/province Ontario
ZIP/Postal code K1H 8M5
Country Canada
 
Platform ID GPL11202
Series (1)
GSE33022 Cardiac aldosterone and corticosterone regulated genes in mice with cardiomyocytes targeted mineralocorticoid receptor overexpression (MR-Cardio mice) and their controls (Ctrl mice)

Data table header descriptions
ID_REF
VALUE normalized log ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
GE_BrightCorner -0.46248344
DarkCorner -0.028654583
A_55_P1989846 -0.58539736
A_55_P1991598 -0.10709185
A_55_P2022211 -1.5526762
A_55_P1980764 1.6370965
A_55_P1964375 0.9434986
A_51_P128876 -1.5363026
A_55_P2121042 -0.121180564
A_52_P219230 -0.7473386
A_51_P207591 -0.11586097
A_55_P2131920 2.6879463
A_55_P2404223 0.7058806
A_55_P2101944 0.102265365
A_52_P358860 -1.454279
A_51_P119031 0.54343235
A_51_P309854 -0.15929985
A_51_P343900 0.073273554
A_51_P234359 -1.7872986
A_51_P487813 -2.0408058

Total number of rows: 39485

Table truncated, full table size 943 Kbytes.




Supplementary file Size Download File type/resource
GSM818520_US23502393_252665510796_S01_GE2_107_Sep09_1_3.txt.gz 4.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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