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Sample GSM815630 Query DataSets for GSM815630
Status Public on Aug 01, 2012
Title heart_dtg_rep4
Sample type RNA
Source name heart
Organism Mus musculus
Characteristics strain: C57BL6
gender: male
genotype/variation: merCremer/+ IGFloxP/IGFloxP
Treatment protocol At the age of 8 month the animals were treated with 4OH-Tamoxifen (500 µg dissolved in 100 µl peanut oil) on 10 consecutive days to excise Exon 3 of the IGF-1R. Six weeks after the treatment hearts were removed for further analysis.
Growth protocol Mice were bred at the Tierversuchsanlage of the Heinrich-Heine-Universitaet, Duesseldorf, Germany. Animal experiments were performed in accordance with the national guidelines on animal care and approved by the Bezirksregierung Duesseldorf. Animals received standard rodent chow diet and tap water ad libitum and were kept on a dark/light cycle of 12/12 h.
Extracted molecule total RNA
Extraction protocol The total RNA was isolated using the Trizol reagent (Invitrogen) following manufactures instructions. After ispropanol precipitation a clean up step using RNeasy Mini columns (Qiagen) were performed.
Label Cy3
Label protocol 100 ng of total RNA were reverse transcribed, linear amplified and labeled using the One Color Low Input Qick Amp Labeling Kit (Agilent).
Hybridization protocol The fragmentated Cy3 labeled cRNA were hybridised on Agilent Mouse GE 4x44K v2 arrays in Hi-RPM Hybridization Buffer (Agilent) at 65°Cand 65 rpm for 17h.
Scan protocol Scannning of the arrays were performed by the Agilent High Resolution Scanner G2505C.
Data processing The fluorescence intensities were calculated with the Feature Extraction Software Version For further data analysis the FE files were imported to GeneSpring GX Version 11.
Submission date Oct 12, 2011
Last update date Aug 01, 2012
Contact name Antje Lindecke
Phone 49-211-8114367
Organization name Universitaet Duesseldorf
Department BMFZ
Lab Microarray
Street address Universitaetsstr. 1
City Duesseldorf
ZIP/Postal code 40225
Country Germany
Platform ID GPL11202
Series (1)
GSE32936 IGF1-receptor signalling attenuates the age-related decline of diastolic cardiac function

Data table header descriptions
VALUE normalized signal

Data table
(-)3xSLv1 -0.07256794
(+)E1A_r60_1 -0.981225
(+)E1A_r60_3 0.5340004
(+)E1A_r60_a104 0.28368545
(+)E1A_r60_a107 0.24102974
(+)E1A_r60_a135 0.07624984
(+)E1A_r60_a20 -0.028695583
(+)E1A_r60_a22 -0.06984615
(+)E1A_r60_a97 -0.57447505
(+)E1A_r60_n11 -0.47490358
(+)E1A_r60_n9 -1.2126331
A_51_P100034 0.15939236
A_51_P100174 0.063408375
A_51_P100208 6.60E-04
A_51_P100289 -0.17310047
A_51_P100298 -0.33990383
A_51_P100309 0.13213468
A_51_P100327 -0.9015403
A_51_P100347 0.02460885
A_51_P100519 -0.04196334

Total number of rows: 39485

Table truncated, full table size 964 Kbytes.

Supplementary file Size Download File type/resource
GSM815630_tg28b_US45103065_252665510194_S01_1_4.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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