|Public on Aug 01, 2012
genotype/variation: merCremer/+ IGFloxP/IGFloxP
|At the age of 8 month the animals were treated with 4OH-Tamoxifen (500 µg dissolved in 100 µl peanut oil) on 10 consecutive days to excise Exon 3 of the IGF-1R. Six weeks after the treatment hearts were removed for further analysis.
|Mice were bred at the Tierversuchsanlage of the Heinrich-Heine-Universitaet, Duesseldorf, Germany. Animal experiments were performed in accordance with the national guidelines on animal care and approved by the Bezirksregierung Duesseldorf. Animals received standard rodent chow diet and tap water ad libitum and were kept on a dark/light cycle of 12/12 h.
|The total RNA was isolated using the Trizol reagent (Invitrogen) following manufactures instructions. After ispropanol precipitation a clean up step using RNeasy Mini columns (Qiagen) were performed.
|100 ng of total RNA were reverse transcribed, linear amplified and labeled using the One Color Low Input Qick Amp Labeling Kit (Agilent).
|The fragmentated Cy3 labeled cRNA were hybridised on Agilent Mouse GE 4x44K v2 arrays in Hi-RPM Hybridization Buffer (Agilent) at 65°Cand 65 rpm for 17h.
|Scannning of the arrays were performed by the Agilent High Resolution Scanner G2505C.
|The fluorescence intensities were calculated with the Feature Extraction Software Version 10.7.3.1. For further data analysis the FE files were imported to GeneSpring GX Version 11.
|Oct 12, 2011
|Last update date
|Aug 01, 2012
|IGF1-receptor signalling attenuates the age-related decline of diastolic cardiac function