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Sample GSM815138 Query DataSets for GSM815138
Status Public on Dec 15, 2011
Title genomic DNA of HCT116 WT cells (r3)
Sample type genomic
Source name Colon
Organism Homo sapiens
Characteristics cell line: HCT116
genotype: wild type
Growth protocol HCT116 cells were maintained in McCoy's 5A medium (Gibco BRL) supplemented with 10% fetal bovine at 37°C under 5% CO2.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted and purified from HCT116 cells using Qiagen QiaAMP Kit according to standard instructions. The recommended RNase treatment was performed.
Label Cy5 and Cy3
Label protocol Standard Infinium HD Methylation Assay protocol
Hybridization protocol Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Infinium HD Methylation Assay protocol.
Scan protocol Arrays were imaged using HiScan Reader using standard recommended Illumina scanner setting.
Description Colorectal cancer cell line
Data processing GenomeStudio software v2010.3, Methylation module v1.8.5
Submission date Oct 12, 2011
Last update date Dec 15, 2011
Contact name Matthieu Defrance
Organization name Université Libre de Bruxelles
Lab Cancer Epigenetics
Street address CP614 route de Lennik 808
City Bruxelles
ZIP/Postal code 1070
Country Belgium
Platform ID GPL13534
Series (1)
GSE29290 Evaluation of the Infinium Methylation 450K technology

Data table header descriptions
VALUE Average Beta
Sample_3.Detection Pval

Data table
ID_REF VALUE Sample_3.Detection Pval
cg00000029 0.8956234 0.00
cg00000108 0.9276204 0.00
cg00000109 0.8725377 0.00
cg00000165 0.8800115 0.00
cg00000236 0.8785453 0.00
cg00000289 0.7327303 0.00
cg00000292 0.8966722 0.00
cg00000321 0.8380153 0.00
cg00000363 0.909277 0.00
cg00000622 0.03005078 0.00
cg00000658 0.8017241 0.00
cg00000714 0.3799543 0.00
cg00000721 0.9059578 0.00
cg00000734 0.1174731 0.00
cg00000769 0.08241253 0.00
cg00000807 0.889397 0.00
cg00000884 0.8306615 0.00
cg00000905 0.179122 0.00
cg00000924 0.4784263 0.00
cg00000948 0.8839747 0.00

Total number of rows: 485577

Table truncated, full table size 12362 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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