|
| Status |
Public on Apr 07, 2024 |
| Title |
90Sr 5 Gy×4, replicate 1, scRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
keloid
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: keloid
cell line: primary
cell type: fibroblast
genotype: GRCh38
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single-cell suspensions at 1 × 105 cells/mL in concentration in PBS were prepared and loaded onto microfluidic devices.
Libraries were constructed according to Singleron GEXSCOPE protocol by GEXSCOPE Single-Cell RNA Library Kit (Singleron Biotechnologies), which included cell lysis, mRNA trapping, labeling cells (barcode) and mRNA (UMI), reverse transcription mRNA into cDNA and amplification, and finally fragment cDNA.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Raw reads were processed to generate gene expression profiles using CeleScope v1.5.2 (Singleron Biotechnologies) with default parameters. Seurat v 3.1.2 was used for quality control, dimensionality reduction and clustering.
Read 1: contains the 24bp GEXSCOPE barcode and the 12bp UMI (bases 25-36). Read 2 contains the cDNA information.
Assembly: GRCh38
Supplementary files format and content: matrix files
|
| |
|
| Submission date |
Mar 07, 2024 |
| Last update date |
Apr 07, 2024 |
| Contact name |
Xiaoqian Li |
| E-mail(s) |
LXQ2267@outlook.com
|
| Organization name |
Sichuan University
|
| Street address |
No.17 People's South Road
|
| City |
Chengdu |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE261116 |
Gene expression profile at single cell level of primary keloid fibroblasts treated with various radiotherapy modalities |
|
| Relations |
| BioSample |
SAMN40301236 |
| SRA |
SRX23874873 |
