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Sample GSM8122628 Query DataSets for GSM8122628
Status Public on Jun 07, 2024
Title Saline biol rep 2
Sample type SRA
 
Source name Brain
Organism Mus musculus
Characteristics strain: C57Bl6/J
Sex: Male
age: 8 weeks
tissue: Brain
genotype: C57BL6/J
treatment: Saline IP
Treatment protocol IP injection of saline or 5mg/kg oxycodone
Growth protocol 8-week old male C57 mice
Extracted molecule total RNA
Extraction protocol Fresh tissue dissection prior to freezing on dry ice
Per 10X Genomics kit instructions
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description 10X Genomics
Data processing cellranger v3.1 'cellranger count'
Filtered cells <=5000 and >=200 unique genes
Filtered cells < 5% mitochondrial reads
Filtered out genes expressed in <3 cells
Filtered data scaled to median number of UMIs and log(x+1) transformed
Assembly: mm10-July2020-A
Supplementary files format and content: hdf5, per-molecule information for all molecules that contain a valid barcode, UI, and were assigned with high confidence to a gene or feature barcode. This is a required file to run cellranger aggr.
 
Submission date Mar 01, 2024
Last update date Jun 07, 2024
Contact name Alexander CW Smith
E-mail(s) smitaw@musc.edu
Phone 843-792-8872
Organization name Medical University of South Carolina
Department Neuroscience
Street address 173 Ashley Ave, BS403
City Charleston
State/province SC
ZIP/Postal code 29425
Country USA
 
Platform ID GPL24247
Series (1)
GSE260687 Single-nuclei characterization of oxycodone's effects on dorsal peduncular nucleus
Relations
BioSample SAMN40216517
SRA SRX23809780

Supplementary file Size Download File type/resource
GSM8122628_928_S3_molecule_info.h5 509.4 Mb (ftp)(http) H5
SRA Run SelectorHelp
Raw data are available in SRA

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